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Aspect ratio has no effect on genotoxicity of multi-wall carbon nanotubes

Aspect ratio has no effect on genotoxicity of multi-wall carbon nanotubes,10.1007/s00204-010-0574-0,Archives of Toxicology,Jin Sik Kim,Kyu Lee,Young H

Aspect ratio has no effect on genotoxicity of multi-wall carbon nanotubes   (Citations: 1)
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Carbon nanotubes (CNTs) have specific physico-chemical and electrical properties that are useful for telecommunications, medicine, materials, manufacturing processes and the environmental and energy sectors. Yet, despite their many advantages, it is also important to determine whether CNTs may represent a hazard to the environment and human health. Like asbestos, the aspect ratio (length:diameter) and metal components of CNTs are known to have an effect on the toxicity of carbon nanotubes. Thus, to evaluate the toxic potential of CNTs in relation to their aspect ratio and metal contamination, in vivo and in vitro genotoxicity tests were conducted using high-aspect-ratio (diameter: 10–15 nm, length: ~10 μm) and low-aspect-ratio multi-wall carbon nanotubes (MWCNTs, diameter: 10–15 nm, length: ~150 nm) according to OECD test guidelines 471 (bacterial reverse mutation test), 473 (in vitro chromosome aberration test), and 474 (in vivo micronuclei test) with a good laboratory practice system. To determine the treatment concentration for all the tests, a solubility and dispersive test was performed, and a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) solution found to be more suitable than distilled water. Neither the high- nor the low-aspect-ratio MWCNTs induced any genotoxicity in a bacterial reverse mutation test (~1,000 μg/plate), in vitro chromosome aberration test (without S9: ~6.25 μg/ml, with S9: ~50 μg/ml), or in vivo micronuclei test (~50 mg/kg). However, the high-aspect-ratio MWCNTs were found to be more toxic than the low-aspect-ratio MWCNTs. Thus, while high-aspect-ratio MWCNTs do not induce direct genotoxicity or metabolic activation–mediated genotoxicity, genotoxicity could still be induced indirectly through oxidative stress or inflammation.
Journal: Archives of Toxicology - ARCH TOXICOL , vol. 85, no. 7, pp. 775-786, 2011
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    • ...Accordingly, to identify the best biocompatible dispersants, we selected Wve dispersants, including distilled water (D.W.), dimethylsulfoxide (DMSO) (Soto et al. 2005; Garza et al. 2008; Di Sotto et al. 2009; Alpatova et al. 2010), a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) solution (Porter et al. 2008; Herzog et al. 2009a, b; Kim et al. 2010a, b; Mercer et al. 2010), 0.5% bovine serum albumin(BSA) (Poland et al. 2008; Bihari et ...
    • ...buVered saline (PBS) (GIBCO, USA), pH 7.4, supplemented with 5.5 mM D-glucose (Sigma, USA) and 0.6 mg/ml species-speciWc serum albumin (GIBCO, USA), 0.01 mg/ml 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) (Sigma, USA) (Porter et al. 2008; Herzog et al. 2009a, b; Kim et al. 2010a, b; Mercer et al. 2010), (4) 0.5% bovine serum albumin(BSA) (GIBCO, USA) in saline (Poland et al. 2008; Bihari et al. 2008; Cheng et al. 2009), and (5) 1% ...
    • ... (Sigma, USA) (Muller et al. 2005; Bihari et al. 2008; Inoue et al. 2008; Takagi et al. 2008), where the respective dispersant concentrations were selected based on previous studies (Soto et al. 2005; Muller et al. 2005; Bihari et al. 2008; Garza et al. 2008; Inoue et al. 2008; Poland et al. 2008; Porter et al. 2008; Takagi et al. 2008; Cheng et al. 2009; Di Sotto et al. 2009; Herzog et al. 2009a, b; Alpatova et al. 2010; Kim et al. 2010a, ...
    • ...Thus, toxicology studies of CNTs have been conducted using such dispersants as a 1,2-dipalmitoylsn-glycero-3-phosphocholine (DPPC) solution, bovine serum albumin (BSA), and Tween 80 (Muller et al. 2005; Bihari et al. 2008; Inoue et al. 2008; Poland et al. 2008; Porter et al. 2008; Takagi et al. 2008; Cheng et al. 2009; Herzog et al. 2009a, b; Mercer et al. 2010; Kim et al. 2010a, b). Porter et al. (2008) already reported that a DPPC ...
    • ... used solvents and dispersants, such as distilled water (D.W.), dimethylsulfoxide (DMSO), a DPPC solution, 0.5% bovine serum albumin (BSA) in saline, and 1% Tween 80 in 0.5% carboxymethylcellulose (Soto et al. 2005; Muller et al. 2005; Bihari et al. 2008; Garza et al. 2008; Inoue et al. 2008; Poland et al. 2008; Porter et al. 2008; Takagi et al. 2008; Cheng et al. 2009; Di Sotto et al. 2009; Herzog et al. 2009a, b; Alpatova et al. 2010; Kim et ...
    • ...However, in our previous study, a cytotoxicity eVect was observed from MWCNTs at a low concentration (0.125% »concentration) when using a lung Xuid mimic surfactant (DPPC solution) (Kim et al. 2010a, b). Also, Herzog et al. (2009a, b) reported that DPPC dispersion inXuences the toxicity of SWCNTs exposure by suppression of IL-8, IL-6, and MCP-1 in A549 cells...

    Jin Sik Kimet al. Evaluation of biocompatible dispersants for carbon nanotube toxicity t...

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