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DNA methylation of the X chromosomes of the human female: an in situ semi-quantitative analysis

DNA methylation of the X chromosomes of the human female: an in situ semi-quantitative analysis,10.1007/BF00352117,Chromosoma,J. Bernardino,E. Lamolia

DNA methylation of the X chromosomes of the human female: an in situ semi-quantitative analysis   (Citations: 12)
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We present an in situ semi-quantitative analysis of the global DNA methylation of the X chromosomes of the human female using antibodies raised against 5-methylcytosine. The antibodies were revealed by immunofluorescence. Images were recorded by a CCD camera and the difference in intensity of fluorescence between active (early replicating) and inactive (late-replicating) X chromosomes was measured. Global hypomethylation of the late-replicating X chromosomal DNA was observed in three cases of fibroblast primary cultures that were characterized by numerical and structural aberrations of the X chromosomes [46,X,ter rea(X;X), 48,XXXX and 46,X,t(X;15)]. In these cases, the difference between early and late-replicating X chromosomes was significantly greater than the intrametaphasic variations, measured for a pair of autosomes, that result from experimental procedures. In cells with normal karyotypes, the differneces between the two X chromosomes were in the range of experimental variation. These results demonstrated that late replication and facultative heterochromatinization of the inactive X are two processes that are not related to global hypermethylation of the DNA
Journal: Chromosoma , vol. 104, no. 7, pp. 528-535, 1996
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    • ...It was not possible to run 5-mC and BrdU analyses simultaneously as a double-colour immunostaining since BrdU incorporation modified the DNA methylation signals, probably due to a changed DNA denaturation profile (see also Bernardino et al., 1996)...

    Jitka Zluvovaet al. Immunohistochemical study of DNA methylation dynamics during plant dev...

    • ...Using the anti-5-mC immunolabelling technique, Barbin et al. (1994) and Bernardino et al. (1996) were not able to find any significant diÄerence between the active and inactive human X-chromosomes...

    Jiří Buet al. Structure and DNA methylation pattern of partially heterochromatinised...

    • ...Using monoclonal antibodies to 5mC (anti-5mC), the sites of methylcytosine-rich DNA were detected on metaphase chromosomes of the normal human lymphocytes and different types of cell lines (Barbin et al. 1994, Montpellier et al. 1994, Bernardino et al. 1996), demonstrating a recurrent pattern of immunofluorescent signals...
    • ...Having studied in situ DNA methylation of chromosomes from human lymphocytes (Barbin et al. 1994, Bernardino et al. 1996), confirming and extending other data (Miller et al. 1974), we examined extraembryonic tissues, obtained after artificial abortions to look for possible alterations in methylation patterns...

    Nadja Kokalj-Vokacet al. DNA methylation of the extraembryonic tissues: an in situ study on hum...

    • ...Xchromosome inactivation has been correlated with both chromosomal hypermethylation (Kerem et al. 1983; Prantera and Ferraro 1990) and hypomethylation (Viegas Pequignot et al. 1988; Miniou et al. 1994; Bernardino et al. 1996)...
    • ...Other studies have failed to demonstrate any difference in methylation between the active and inactive X chromosome (Adolph and Hameister 1990; Sumner et al. 1990; de la Torre et al. 1992; Barbin et al. 1994; Miniou et al. 1994; Bernardino et al. 1996)...
    • ...The replication status of X chromosomes has been determined through incorporation of bromodeoxyuridine into late-replicating DNA (Kerem et al. 1983; Prantera and Ferraro 1990; Barbin et al. 1994; Miniou et al. 1994; Bernardino et al. 1996)...

    Claus Lindbjerg Andersenet al. CpG islands detected by self-primed in situ labeling (SPRINS)

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