Academic
Publications
The Influence of Fluoride Exposure on Dentin Mineralization Using an in Vitro Organ Culture Model

The Influence of Fluoride Exposure on Dentin Mineralization Using an in Vitro Organ Culture Model,10.1007/s00223-003-0022-8,Calcified Tissue Internati

The Influence of Fluoride Exposure on Dentin Mineralization Using an in Vitro Organ Culture Model   (Citations: 6)
BibTex | RIS | RefWorks Download
This study aimed to characterize fluoride-induced alterations in dentin mineralization within a dentin-pulp organ culture system. Tooth sections derived from male Wistar rat incisors were cultured in Trowel-type culture for 14 days, in the presence of 0 mM, 1 mM, 3 mM and 6 mM sodium fluoride. Tooth sections were processed and analyzed for uptake of fluoride, its subsequent effect on dentin mineralization by tetracycline hydrochloride incorporation and mineral composition, expressed as calcium/phosphorous (Ca/P) ratios. Tetracycline hydrochloride incorporation was demonstrated to decrease with increased fluoride exposure, accompanied by significant increases in both Ca/P ratios and fluoride incorporation. These findings provide further evidence that the established alterations in dentin formation during fluorosis are a consequence of disruption to the mineralization process, and provide a model system with which to investigate further the potential role the extracellular matrix plays in inducing the apparent changes in mineral composition.
Journal: Calcified Tissue International - CALCIFIED TISSUE INT , vol. 73, no. 5, pp. 470-475, 2003
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
Sort by: