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Novel embryonic regulation of Ca 2+ -activated K + channel expression in Drosophila

Novel embryonic regulation of Ca 2+ -activated K + channel expression in Drosophila,10.1007/BF02211941,Invertebrate Neuroscience,Tarita Thomas,Bin Wan

Novel embryonic regulation of Ca 2+ -activated K + channel expression in Drosophila   (Citations: 5)
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Theslowpoke gene ofDrosophila melanogaster encodes a Ca2+-activated K+ channel that is expressed in neurons, muscles, tracheal cells and the middle midgut. The entire transcriptional control region ofslowpoke is contained in 11 kb of genomic DNA. Previous work has identified four different tissue-specific promoters (Promoters C1, C1b, C1c and C2) and sequences that regulate their activity. Here we describe and contrast the regulation of neuronal and muscle expression during embryogenesis with its regulation during larval and adult stages. Embryonic regulation is fundamentally different. The embryo uses Promoter C1 and a previously undescribed promoter, called Promoter Ce, to drive neuronal expression. The expression patterns of these promoters are distinct. Muscle expression arises from Promoter C2 as in other developmental stages. A downstream intronic region has been shown to contain control elements that modulate promoter activity differently in embryos, larvae and adults. Embryonic CNS expression is not dependent on the intron, however; its deletion has substantial effects on neuronal expression in larvae and adults. In embryonic muscle, removal of the intron eliminates muscle expression even though this deletion does not reduce larval muscle expression.
Journal: Invertebrate Neuroscience - INVERTEBR NEUROSCI , vol. 2, no. 4, pp. 283-291, 1997
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    • ...Not shown are the names of other alternative exons that result from differential promoter utilization, which have been called C1, C1b, C1c, and C2 (Becker et al., 1995; Brenner and Atkinson, 1996, 1997; Brenner et al., 1996; Thomas et al., 1997)...
    • ...The slowpoke transcriptional control region has at least five distinct transcriptional promoters (Brenner and Atkinson, 1996; Thomas et al., 1997)...
    • ...This control region has been well characterized (Becker et al., 1995; Brenner and Atkinson, 1996, 1997; Brenner et al., 1996; Thomas et al., 1997) and mimics the expression pattern of the endogenous gene...
    • ...This DNA contains all five of the slowpoke tissue-specific transcriptional promoters (Fig. 1 and Thomas et al., 1997), each of which generates a unique 59 exon...

    Robert Brenneret al. Complementation of Physiological and Behavioral Defects by a Slowpoke ...

    • ...Separate promoters for neural and midgut expression were identified, while muscle and tracheal cell expression were shown to arise from a shared promoter (Brenner and Atkinson, 1996; Brenner et al., 1996; Thomas et al., 1997)...
    • ...The construction of P12 has been described by Thomas et al. (1997)...
    • ...Thomas et al. (1997) provided evidence for a second neuronal promoter by deletion analysis...

    RUDOLF A. BOHMet al. TRANSCRIPTIONAL CONTROL OF Ca2+ACTIVATED K+ CHANNEL EXPRESSION: IDENTI...

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