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An on-column derivatization method for the determination of homocysteine-thiolactone and protein N -linked homocysteine

An on-column derivatization method for the determination of homocysteine-thiolactone and protein N -linked homocysteine,10.1007/s00726-010-0521-7,Amin

An on-column derivatization method for the determination of homocysteine-thiolactone and protein N -linked homocysteine   (Citations: 4)
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Homocysteine (Hcy) is incorporated into protein via a reaction of the thioester Hcy-thiolactone with ε-amino group of a protein lysine residue generating N-Hcy-protein. This reaction impairs and alters protein’s function and has been implicated in atherothrombotic disease. Here, we describe new high-performance liquid chromatography assays for the determination of Hcy-thiolactone, protein N-linked Hcy, and Hcy based on an on-column derivatization with o-phthaldialdehyde and fluorescence detection. The on-column derivatization generates narrow peaks, which allows fast run times (3–5 min) and facilitates determination of N-linked Hcy directly from acid hydrolysates of plasma protein. Utility of these assays was demonstrated with human urine and plasma samples.
Journal: Amino Acids , vol. 41, no. 1, pp. 187-194, 2011
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    • ...Recently, analysis of trace AA in physiological fluids have received more attention (Badawy et al. 2008; Suryawan et al. 2009; Gowacki et al. 2010a) because the analysis of these compounds could provide fundamental and important information for medical, biological, and clinical fields...
    • ...For example, the determination of physiological AA is of importance not only to the clinical diagnosis and treatment of hereditary diseases (Berson et al. 1976), liver diseases (Cascino et al. 1978), neuropathy (Zeman et al. 2008), diabetes (Carlsten et al. 2009), atherothrombotic disease (Gowacki et al. 2010a), etc., but also to the evaluation of the nutritive status and normal reference of the patient and breast-fed infants (Lindblad et ...

    Guoliang Liet al. Determination of trace amino acids in human serum by a selective and s...

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