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Heterologous production of Pseudomonas aeruginosa EMS1 biosurfactant in Pseudomonas putida

Heterologous production of Pseudomonas aeruginosa EMS1 biosurfactant in Pseudomonas putida,10.1016/j.biortech.2007.05.035,Bioresource Technology,Misun

Heterologous production of Pseudomonas aeruginosa EMS1 biosurfactant in Pseudomonas putida   (Citations: 8)
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A new bacterial strain isolated from activated sludge, identified as Pseudomonas aeruginosa EMS1, produced a biosurfactant when grown on acidified soybean oil as the sole carbon source. An optimum biosurfactant production of 5g/L was obtained with the following medium composition: 2% acidified soybean oil, 0.3% NH4NO3, 0.03% KH2PO4, 0.03% K2HPO4, 0.02% MgSO4·7H2O and 0.025% CaCl2·2H2O, with shaking at 200rpm for an incubation period of 100h at 30°C. The production of the biosurfactant was found to be a function of cell growth, with maximum production occurring during the exponential phase. Hemolysis of erythrocytes and thin-layer chromatography studies revealed that the secreted biosurfactant was rhamnolipid.To overcome the complex environmental regulation with respect to rhamnolipid biosynthesis, and to replace the opportunistic pathogen P. aeruginosa with a safe industrial strain, attempts were made to achieve rhamnolipid production in a heterologous host, Pseudomonas putida, using molecular cloning of rhlAB rhamnosyltransferase genes with the rhlRI quorum sensing system, assuming that a functional rhamnosyltransferase would catalyze the formation of rhamnosyl-6-hydroxydecanoyl-6-hydroxydecanoate (mono-rhamnolipid) in P. putida. It was shown that rhamnolipid can be produced in the heterologous strain, P. putida, when provided with the rhamnosyltransferase genes.
Journal: Bioresource Technology - BIORESOURCE TECHNOL , vol. 99, no. 7, pp. 2192-2199, 2008
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