Enzyme immobilization on thermosensitive hydrogel microspheres

Enzyme immobilization on thermosensitive hydrogel microspheres,10.1016/0927-7765(94)01177-7,Colloids and Surfaces B-biointerfaces,Toshifumi Shiroya,No

Enzyme immobilization on thermosensitive hydrogel microspheres   (Citations: 18)
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Precipitation polymerization of N-isopropylacrylamide, acrylamide, and methylenebisacrylamide in water at 70°C resulted in thermosensitive hydrogel microspheres. Carboxyl groups on the microspheres were introduced by hydrolysis, and amino groups by the Hofmann reaction of amide units on the microspheres. Trypsin was immobilized on the carboxylated microspheres using carbodiimide.Phase transitions were detected using a hydrophobic fluorescence probe. The temperatures at which a phase transition occurred were increased by immobilizing enzymes. The enzymatic activity of the immobilized enzymes decreased above the transition temperature. This was attributed to (i) a decrease in the diffusion of substrate; and (ii) entrapment of enzyme in the surface layer of the microspheres. In an attempt to overcome the entrapment, enzymes were immobilized via a hydrophilic spacer (α-(carboxymethyl)-ω-(carboxymethoxy)-poly(oxy-1,2-ethanediyl), PEO acid) to the microspheres. These enzyme-carrying hydrogel microspheres were found to show an enzymatic activity independent of temperature, even though these conjugates show a phase transition at the lower critical solution temperature.
Journal: Colloids and Surfaces B-biointerfaces - COLLOID SURFACE B , vol. 4, no. 5, pp. 267-274, 1995
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    • ...Separation (Cussler et al., 1984; Freitas and Cussler, 1987) Bioreaction (Park and Hoffman, 1993) Shape memory (Hu et al., 1995) Artificial muscle (Kishi et al., 1993) Enzyme immobilization (Shiroya et al., 1995) pH Drug delivery (Bala and Vasudevan, 1982; Brazel and Peppas, 1996; Dong and Hoffman, 1991) Electric field Drug delivery (Kwon et al., 1991; Sawahata et al., 1990)...

    Jung Ju Kim. Smart hydrogels for bioseparation

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