Numerous adrenal chromaffin cell preparations fail to produce analgesic effects in the formalin test or in tests of acute pain even with nicotine stim

Numerous adrenal chromaffin cell preparations fail to produce analgesic effects in the formalin test or in tests of acute pain even with nicotine stimulation (Citations: 11)


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Previous studies have reported that intrathecal implants of a variety of adrenal chromaffin cell preparations all produce analgesic effects in rodents. The major objective of the present study was to determine if any adrenal chromaffin cell preparations produce more robust analgesic effects than other cell preparations. The present study included adult rat adrenal chromaffin tissue allografts, purified adult bovine chromaffin cells, and polymer-encapsulated calf adrenal chromaffin cells, all prepared according to previously published procedures, as well as purified calf adrenal chromaffin cells. Previous studies have also suggested that immunosuppression may play a role in graft survival, and potentially increase the magnitude of analgesic effects, so the present study included both immunosuppressed and non-immunosuppressed groups (cyclosporin A, 10 mg/kg per day). Behavioral tests included the formalin test; and a dorsal tail-flick, hot-plate, and paw-pinch test, conducted sequentially 2 min after systemic nicotine (0.1 mg/kg) to evoke release from the chromaffin cells, as previously reported. Analgesic effects related to morphine and nicotine were detected, and consistent differences in performance could be detected between individual animals. Surprisingly, no analgesic effects were detectable with any of the four chromaffin cell preparations, with or without immunosuppression, in the formalin test or with nicotine stimulation in tests of acute pain.

Journal: Pain , vol. 88, no. 2, pp. 177-188, 2000

DOI: 10.1016/S0304-3959(00)00324-9

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Keywords (5)

Numerous adrenal chromaffin cell preparations fail to produce analgesic effects in the formalin test or in tests of acute pain even with nicotine stim

References (24)

Bovine chromaffin cells for CNS transplantation do not elicit xenogeneic T cell proliferative responses in vitro (Citations: 9)

Intrathecal implants of bovine chromaffin cells alleviate mechanical allodynia in a rat model of neuropathic pain (Citations: 38)

Subarachnoid spinal cord transplantation of adrenal medulla suppresses chronic neuropathic pain behavior in rats (Citations: 24)

Reduced pain-related behavior by adrenal medullary transplants in rats with experimental painful peripheral neuropathy (Citations: 41)

Alleviation of neuropathic pain symptoms by xenogeneic chromaffin cell grafts in the spinal subarachnoid space (Citations: 33)

Citations (11)

Reduction of Allodynia by Intrathecal Transplantation of Microencapsulated Porcine Chromaffin Cells (Citations: 3)

DEAFFERENTATION PAIN RESULTING FROM CERVICAL POSTERIOR RHIZOTOMY IS ALLEVIATED BY CHROMAFFIN CELL TRANSPLANTS INTO THE RAT SPINAL SUBARACHNOID SPACE (Citations: 3)

Intrathecal xenogeneic chromaffin cell grafts reduce nociceptive behavior in a rodent tonic pain model (Citations: 7)

Enhanced antinociception by nicotinic receptor agonist epibatidine and adrenal medullary transplants in the spinal subarachnoid space (Citations: 1)

Huntington's disease: pre-clinical models to therapy using genetically-modified immunoisolated cells and lentiviral vectors


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