Academic
Publications
Protein kinase inhibitors substantially improve the physical detection of T-cells with peptide-MHC tetramers

Protein kinase inhibitors substantially improve the physical detection of T-cells with peptide-MHC tetramers,10.1016/j.jim.2008.09.014,Journal of Immu

Protein kinase inhibitors substantially improve the physical detection of T-cells with peptide-MHC tetramers   (Citations: 11)
BibTex | RIS | RefWorks Download
Anna Lissina, Kristin Ladell, Ania Skowera, Matthew Clement, Emily Edwards, Ruth Seggewiss, Hugo A. van den Berg, Emma Gostick, Kathleen Gallagher, Emma Jones, J. Joseph Melenhorst, Andrew J. Godkinhttp://academic.research.microsoft.com/io.ashx?type=5&id=35728533&selfId1=0&selfId2=0&maxNumber=12&query=
Flow cytometry with fluorochrome-conjugated peptide-major histocompatibility complex (pMHC) tetramers has transformed the study of antigen-specific T-cells by enabling their visualization, enumeration, phenotypic characterization and isolation from ex vivo samples. Here, we demonstrate that the reversible protein kinase inhibitor (PKI) dasatinib improves the staining intensity of human (CD8+ and CD4+) and murine T-cells without concomitant increases in background staining. Dasatinib enhances the capture of cognate pMHC tetramers from solution and produces higher intensity staining at lower pMHC concentrations. Furthermore, dasatinib reduces pMHC tetramer-induced cell death and substantially lowers the T-cell receptor (TCR)/pMHC interaction affinity threshold required for cell staining. Accordingly, dasatinib permits the identification of T-cells with very low affinity TCR/pMHC interactions, such as those that typically predominate in tumour-specific responses and autoimmune conditions that are not amenable to detection by current technology.
Journal: Journal of Immunological Methods - J IMMUNOL METHOD , vol. 340, no. 1, pp. 11-24, 2009
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
Sort by: