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Dried tube specimens: A simple and cost-effective method for preparation of HIV proficiency testing panels and quality control materials for use in resource-limited settings

Dried tube specimens: A simple and cost-effective method for preparation of HIV proficiency testing panels and quality control materials for use in re

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Dried tube specimens: A simple and cost-effective method for preparation of HIV proficiency testing panels and quality control materials for use in resource-limited settings   (Citations: 2)
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HIV testing has rapidly expanded worldwide, but proficiency testing (PT) programs to monitor and improve the quality of testing are often lacking in resource-limited settings (RLS). Traditional PT programs and quality control reagents use serum or plasma specimens requiring stringent conditions for storage and transportation.A novel, simple and easy to use approach, based on dried tube specimens (DTS), was developed that can help monitor the quality of HIV antibody testing in RLS. DTS were prepared by drying 20μl of specimen overnight at room temperature. The addition of a green dye (0.1%) made the DTS pellets visible without affecting the test results. Before testing, the DTS were rehydrated with 200μl of PBS–Tween buffer. A panel of 303 DTS samples (135 HIV positive and 168 HIV negative) was evaluated with two rapid tests. Sensitivity and specificity with the Determine HIV-1/2 test were 99.3% and 99.4%, respectively, and with OraQuick were 98.5% and 100%, respectively. Stability studies showed that HIV-specific antibodies in the DTS specimens were stable at 4°C and 25°C for 4 weeks, with only marginal decline at 37°C and 45°C over 4 weeks. The DTS-based PT program was piloted successfully in 24 testing sites in Kenya.Results demonstrate that the DTS is a simple to use, practical method to prepare and distribute PT panels and quality control specimens to monitor HIV testing practices in RLS.
Journal: Journal of Virological Methods - J VIROL METH , vol. 163, no. 2, pp. 295-300, 2010
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    • ...For quality assurance, supervisors were provided with proficiency panels prepared by a reference laboratory, consisting of tubes in which sera characterized as positive or negative had been allowed to dry at room temperature. Dried sera were reconstituted with buffered saline on site and used to assess the performance of POCTs by HCWs. This simple, low-cost method was originally developed to assure the quality of HIV testing ...

    David C. Mabey, et al. Point-of-Care Tests to Strengthen Health Systems and Save Newborn Live...

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