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The chimpanzee Mhc-DRB region revisited: Gene content, polymorphism, pseudogenes, and transcripts

The chimpanzee Mhc-DRB region revisited: Gene content, polymorphism, pseudogenes, and transcripts,10.1016/j.molimm.2009.09.003,Molecular Immunology,Na

The chimpanzee Mhc-DRB region revisited: Gene content, polymorphism, pseudogenes, and transcripts   (Citations: 2)
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In humans, great apes, and different monkey species, the major histocompatibility complex (MHC) class II DRB region is known to display considerable copy number variation. The microsatellite D6S2878 has been shown to be a valuable marker for haplotyping the DR region in humans and macaque species. The present report illustrates that chimpanzee haplotypes also can be discriminated with this marker. The analyses resulted in the description of nine different region configurations, of which seven are present within the West African chimpanzee population studied. The region configurations vary in gene content from two up to five DRB genes. Subsequent cDNA sequencing increased the number of known full-length Patr-DRB sequences from 3 to 32, and shows that one to three Patr-DRB genes per haplotype apparently produce functional transcripts. This is more or less comparable to humans and rhesus macaques. Moreover, microsatellite analysis in concert with full-length DRB gene sequencing showed that the Patr-DRB*W9 and -DRB3*01/02 lineages most likely arose from a common ancestral lineage: hence, the Patr-DRB*W9 lineage was renamed to Patr-DRB3*07. Overall, the data demonstrate that the D6S2878 microsatellite marker allows fast and accurate haplotyping of the Patr-DRB region. In addition, the limited amount of allelic variation observed at the various Patr-DRB genes is in agreement with the fact that chimpanzees experienced a selective sweep that may have been caused by an ancient retroviral infection.
Journal: Molecular Immunology - MOL IMMUNOL , vol. 47, no. 2, pp. 381-389, 2009
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    • ...Closely linked markers, such as microsatellites, have already been successfully used for characterization of the whole MHC region (Penedo et al. 2005; Wiseman et al. 2007; Wojcechowskyj et al. 2007), the B (Bonhomme et al. 2008; Doxiadis et al. 2009b), and the DR region in different macaque and other primate species (Doxiadis et al. 2007, 2009a, 2010; de Groot et al. 2008, 2009)...

    Gaby G. M. Doxiadiset al. Genomic plasticity of the MHC class I A region in rhesus macaques: ext...

    • ...In humans, the number of DRB loci per haplotype varies from one to four and five major region configurations with different gene numbers and content are known (DR8, DR1, DR51, DR52, and DR53), whereas in chimpanzees nine and in rhesus macaques more than 30 region configurations have been defined with up to five and six DRB loci per haplotype, respectively (Mayer et al. 1992; Khazand et al. 1999; Doxiadis et al. 2007; de Groot et al. 2009)...
    • ...Genotyping of panels of humans (Doxiadis et al. 2007, 2009), chimpanzees (de Groot et al. 2009), and rhesus (Doxiadis et al. 2007) and cynomolgus macaques (de Groot et al. 2008) allowed the definition of unique haplotyping patterns in all four species...
    • ...In humans, only five major DRB region configurations are known and designated, DR8, DR1, DR51, DR52, and DR53, (Marsh et al. 2005), whereas in chimpanzees nine (de Groot et al. 2009), and in rhesus macaques, mostly of Indian origin, about 30 different configurations have been defined (Slierendregt et al. 1994; Khazand et al. 1999; Doxiadis et al. 2000)...
    • ...In chimpanzees, a species older than humans in evolutionary terms, the intermediate situation can be observed, since there exists at least one haplotype without a DRB1 gene, and it is plausible that DRB3 and/or DRB4 have taken over its function (de Groot et al. 2009)...

    Gaby G. M. Doxiadiset al. Extensive DRB region diversity in cynomolgus macaques: recombination a...

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