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Presynaptic Association of Rad51 Protein with Selected Sites in Meiotic Chromatin

Presynaptic Association of Rad51 Protein with Selected Sites in Meiotic Chromatin,10.1073/pnas.93.12.5920,Proceedings of The National Academy of Scien

Presynaptic Association of Rad51 Protein with Selected Sites in Meiotic Chromatin   (Citations: 45)
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Eukaryotic homologs of Escherichia coli RecA protein have been shown to form nucleoprotein filaments with single-stranded DNA that recognize homologous sequences in duplex DNA. Several recent reports in four widely diverse species have demonstrated the association of RecA homologs with meiotic prophase chromatin. The current immunocytological study on mouse spermatocytes and oocytes shows that a eukaryotic homolog, Rad51, associates with a subset of chromatin sites as early as premeiotic S phase, hours before either the appearance of precursors of synaptonemal complexes or the initiation of synapsis. When homologous chromosomes do begin to pair, the Rad51-associated sequences are sites of initial contact between homologues and of localized DNA synthesis. Distribution of Rad51 foci on the chromatin of fully synapsed bivalents at early pachynema corresponds to an R-band pattern of mitotic chromosomes. R-bands are known to be preferred sites of both synaptic initiation and recombination. The time course of appearance of Rad51 association with chromatin, its distribution, and its interaction with other Rad51-associated sequences suggests that it plays an important role preselection of sequences and synaptic initiation.
Journal: Proceedings of The National Academy of Sciences - PNAS , vol. 93, no. 12, pp. 5920-5924, 1996
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