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A Cyclopentenone Prostaglandin Activates Mesangial MAP Kinase Independently of PPARγ

A Cyclopentenone Prostaglandin Activates Mesangial MAP Kinase Independently of PPARγ,10.1006/bbrc.2001.4301,Biochemical and Biophysical Research Commu

A Cyclopentenone Prostaglandin Activates Mesangial MAP Kinase Independently of PPARγ   (Citations: 30)
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The mitogen-activated protein (MAP) kinases mediate the response of renal glomerular mesangial cells to a variety of physiologic and pathologic stimuli. This investigation examines the effect of the cyclopentenone prostaglandin 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) on MAP kinases in human mesangial cells. We show that 15d-PGJ2 dose-dependently increases the extracellular signal-regulated kinase (ERK) activity of human mesangial cells, but has no effect on Jun-NH2-terminal kinase or p38 MAP kinase. Despite the fact that 15d-PGJ2 is a peroxisome proliferator-activated receptor (PPAR) ligand, and PPARγ is shown to be expressed by mesangial cells, the thiazolidinedione PPARγ agonist ciglitazone does not activate ERK. Additionally, a synthetic PPARγ antagonist does not attenuate the activation of ERK by 15d-PGJ2. 15d-PGJ2-mediated ERK activation is however blocked by the MEK inhibitor PD 098059, appears to require phosphatidylinositol-3 kinase, but is independent of protein kinase C activation. These results demonstrate a novel effect of 15d-PGJ2 to induce ERK in human mesangial cells independently of PPARγ.
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