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Applications of direct detection device in transmission electron microscopy

Applications of direct detection device in transmission electron microscopy,10.1016/j.jsb.2007.10.007,Journal of Structural Biology,Liang Jin,Anna-Cla

Applications of direct detection device in transmission electron microscopy   (Citations: 8)
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A prototype direct detection device (DDD) camera system has shown great promise in improving both the spatial resolution and the signal to noise ratio for electron microscopy at 120–400keV beam energies (Xuong et al., 2007. Methods in Cell Biology, 79, 721–739). Without the need for a resolution-limiting scintillation screen as in the charge coupled device (CCD), the DDD camera can outperform CCD based systems in terms of spatial resolution, due to its small pixel size (5μm). In this paper, the modulation transfer function (MTF) of the DDD prototype is measured and compared with the specifications of commercial scientific CCD camera systems. Combining the fast speed of the DDD with image mosaic techniques, fast wide-area imaging is now possible. In this paper, the first large area mosaic image and the first tomography dataset from the DDD camera are presented, along with an image processing algorithm to correct the specimen drift utilizing the fast readout of the DDD system.
Journal: Journal of Structural Biology - J STRUCT BIOL , vol. 161, no. 3, pp. 352-358, 2008
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