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Microwave-assisted extraction of human hair proteins

Microwave-assisted extraction of human hair proteins,10.1016/j.ab.2010.08.021,Analytical Biochemistry,Seongjae Shin,Aera Lee,Sunyoung Lee,KyungJun Lee

Microwave-assisted extraction of human hair proteins  
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The effectiveness of microwave-assisted extraction of proteins from human hair samples was evaluated. Extractions were performed from 2-mg hair samples in an extraction solution consisting of 25mM Tris–HCl (pH 8.5), 2.6M thiourea, 5M urea, and 5% mercaptoethanol. During extraction, samples were exposed to microwave radiation (600W) for a specified incubation period (5–120min). The extraction efficiency of samples that had been incubated for 60min was similar to that of samples that had been heated at 50°C for 24h using the conventional Shindai method.
Journal: Analytical Biochemistry - ANAL BIOCHEM , vol. 407, no. 2, pp. 281-283, 2010
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