Academic
Publications
Plasmodesmal targeting and intercellular movement of potato mop-top pomovirus is mediated by a membrane anchored tyrosine-based motif on the lumenal side of the endoplasmic reticulum and the C-terminal transmembrane domain in the TGB3 movement protein

Plasmodesmal targeting and intercellular movement of potato mop-top pomovirus is mediated by a membrane anchored tyrosine-based motif on the lumenal s

Plasmodesmal targeting and intercellular movement of potato mop-top pomovirus is mediated by a membrane anchored tyrosine-based motif on the lumenal side of the endoplasmic reticulum and the C-terminal transmembrane domain in the TGB3 movement protein   (Citations: 5)
BibTex | RIS | RefWorks Download
Live-cell fluorescence microscopy was used to investigate the third triple gene block protein (TGB3) of potato mop-top pomovirus and its role in assisted targeting of TGB2 to plasmodesmata (PD). Wild-type and mutant TGB3 proteins were expressed under the control of the 35S promoter or from a virus reporter clone. Assisted targeting of TGB2 to PD was optimal when the proteins were expressed from a bicistronic plasmid in the relative ratios expected in a virus infection, suggesting that excess TGB3 inhibited PD localisation. Contrary to the generally accepted view, bimolecular fluorescence complementation showed that the TGB3 N terminus is located in the cytosol. Mutational analysis to dissect TGB3 sub domain functions showed that PD targeting was mediated by a composite signal comprising an ER-lumenal tyrosine-based motif and the C-terminal transmembrane domain. Mutation of either of these domains also abolished cell-to-cell movement of the virus. The results are discussed in the context of TGB3 membrane topology.
Journal: Virology , vol. 402, no. 1, pp. 41-51, 2010
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
    • ...In addition, plasmodesmal targeting and intercellular trafficking of Potato mop-top pomovirus (PMTV) are mediated by a composite signal that consists of an ER-lumenal tyrosinebased motif and the C-terminal transmembrane domain of TGBp3 (Tilsner et al. 2010)...
    • ...This finding indicates that the mechanism of delivery of viral RNPs to PD may differ between viruses with class 1 TGBps including hordei-, pomo-, peclu-, and benyviruses (Tilsner et al. 2010)...

    Tae Kyung Hyunet al. Cell-to-cell trafficking of RNA and RNA silencing through plasmodesmat...

Sort by: