Array-based profiling of ragweed and mugwort pollen allergens

Array-based profiling of ragweed and mugwort pollen allergens,10.1111/j.1398-9995.2008.01780.x,Allergy,G. Gadermaier,N. Wopfner,M. Wallner,M. Egger,A.

Array-based profiling of ragweed and mugwort pollen allergens   (Citations: 12)
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Journal: Allergy , vol. 63, no. 11, pp. 1543-1549, 2008
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    • ... but especially quantitative conclusions concerning the risk level. This study was also conducted prospectively, ensuring more reliable definition and scoring of the severity of symptoms. One hundred and forty-eight patients, living in the same region, were classified into 4 grades of peach oral allergy syndrome (OAS): grade I (isolated OAS), grade II (OAS + gastrointestinal symptoms), grade III (OAS + systemic symptoms, urticaria, angioedema, rhinoconjunctivitis, asthma) and grade IV (OAS + life-threatening symptoms, glottis edema, hypotension, syncope). Grade I patients with so-called mild symptoms were compared to grade II + III + IV patients with so-called severe symptoms. As patients of this geographical region were also exposed to birch pollen, specific plasma IgE assays were performed for recombinant Pru p 1, Pru p 3, Pru p 4, Bet v 1, Bet v 2 and Bet v 4. One of the most important findings was that skin reactivity for peach lipid transfer protein (LTP) was positively correlated with anti-rPru p 3 IgE and negatively correlated with anti-rPru p 1 IgE. A significantly higher percentage of subjects with grade I OAS presented sensitization to birch, grasses, wall pellitory and ragweed, but not mugwort. Eighty-seven percent of patients with severe OAS were sensitized to Pru p 3, i.e. 6 times more often than grade I patients. An inverse proportion was observed for sensitization to birch. The anti-Pru p 1, anti-Pru p 4, anti-Bet v 1 and anti-Bet v 2 IgE concentrations were significantly higher in grade I patients, while the anti-Pru p 3 IgE concentration was significantly higher in patients with severe OAS. A negative correlation was observed between anti-Pru p 1 IgE and anti-Pru p 3 IgE, but also with the severity of symptoms. A 1 kUA/l increase of anti-Pru p 1 corresponded to a 2.5% reduction in the odds of developing severe OAS, but the same 1 kUA/l increase of anti-Pru p 3 IgE corresponded to a 9% increase in the odds of developing severe OAS. Analysis of sensitivity and specificity indicated that a value of 2.69 kUA/l of anti-rPru p 3 discriminated peach-allergic patients at risk for developing more severe symptoms, but antipeach IgE values did not reach a similar positive cutoff. As Pru p 3 is an LTP resistant to gastric enzymes, it would be useful to verify the exact nature of the sensitization of the 19 patients with grade II OAS, as their gastrointestinal reactivity must be considered to be a local phenomenon and not a systemic reaction.In this context, as local specific immunotherapy has been validated for the sublingual-swallow method but not for the sublingual-spit method, anti-LTP IgE assay could be useful to explain and prevent adverse effects of local specific immunotherapy. This predictive role needs to be verified case by case due to the limited cross-reactivity between mugwort Art v 3 and ragweed Amb a 6, explained by the low sequence identity (32%) between these two LTPs [...

    Claude André. Predictive Surrogate Markers of Allergy: A By-Product of Recombinant A...

    • ...Example: Specific immunotherapy in birch pollen allergy: Bet v 1 sensitization or not? Insect venom allergy: sensitization to bee or wasp [37, 38]? House dust mite allergy: sensitization to Der p t or Der f a? Ragweed or mugwort sensitization [57]?...

    Kirsten Skamstrup Hansenet al. Component Resolved Testing for Allergic Sensitization

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