Cripto-1 induces apoptosis in HC11 mouse mammary epithelial cells

Cripto-1 induces apoptosis in HC11 mouse mammary epithelial cells,10.1038/sj.cdd.4400588,Cell Death and Differentiation,M L De Santis,I Martinez-Lacac

Cripto-1 induces apoptosis in HC11 mouse mammary epithelial cells   (Citations: 5)
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Cripto-1 (CR-1) is an epidermal growth factor (EGF)-related protein. CR-1 can inhibit β-casein and whey acidic protein expression in mouse mammary epithelial cells. The present study demonstrates that CR-1 can induce apoptosis in HC-11 mouse mammary epithelial cells, as measured by bis-benzimide stained nuclei, TUNEL assay and cell death ELISA. Apoptosis could be observed after 2 days of exposure of confluent HC-11 cells to CR-1 in the absence of the survival factors EGF and insulin, with maximum apoptosis occurring at 3 days. A reduction in poly(ADP-ribose) polymerase (PARP) expression and an increase in β-catenin cleavage was found after 18 h of exposure to CR-1 suggesting that apoptosis was preceded by the induction of a caspase activity since the caspase inhibitor ZFAD.FMK could block the CR-1-induced reduction in PARP expression and CR-1-induced apoptosis. CR-1 was found to increase the expression of caspase-3-like protease. Although, the levels of p27kip1 and p21Bax did not change after exposure to CR-1 for 18 h, the levels of Bcl-xL became undetectable. These studies suggest that CR-1 promotes apoptosis by mediating the induction of caspase-3-like protease and downregulating the expression of Bcl-xL
Journal: Cell Death and Differentiation - CELL DEATH DIFFERENTIATION , vol. 7, no. 2, pp. 189-196, 2000
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    • ...This may be the case since it has recently been demonstrated that CR-1 can induce apoptosis in confluent, survival-factor depleted HC-11 cells [36]...
    • ...This effect is mediated through an increase in the expression of a caspase-3-like protease and a downregulation in the level of Bcl-xl [36]...

    Caterina Biancoet al. Identification of Cripto-1 in human milk

    • ...Signaling pathways from many hormones may converge to finely control gene expression; e.g., EGF has been found to regulate steady-state levels of bcl-x RNA in mammary cancer cells (13), and Cripto-1 can reduce levels of bcl-x RNA in HC11 cells (14)...

    Kristel Schorret al. Induction of bcl-xL Expression in Mammary Epithelial Cells Is Glucocor...

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