Author | Conference | Journal | Organization | Year | DOI
Look for results that meet for the following criteria:
Academic
Publications
Chemical genomics in Escherichia coli identifies an inhibitor of bacterial lipoprotein targeting

Chemical genomics in Escherichia coli identifies an inhibitor of bacterial lipoprotein targeting,10.1038/nchembio.221,Nature Chemical Biology,Ranjana

Edit
Chemical genomics in Escherichia coli identifies an inhibitor of bacterial lipoprotein targeting   (Citations: 9)
BibTex | RIS | RefWorks Download
One of the most significant hurdles to developing new chemical probes of biological systems and new drugs to treat disease is that of understanding the mechanism of action of small molecules discovered with cell-based small-molecule screening. Here we have assembled an ordered, high-expression clone set of all of the essential genes from Escherichia coli and used it to systematically screen for suppressors of growth inhibitory compounds. Using this chemical genomic approach, we demonstrate that the targets of well-known antibiotics can be identified as high copy suppressors of chemical lethality. This approach led to the discovery of MAC13243, a molecule that belongs to a new chemical class and that has a unique mechanism and promising activity against multidrug-resistant Pseudomonas aeruginosa. We show that MAC13243 inhibits the function of the LolA protein and represents a new chemical probe of lipoprotein targeting in bacteria with promise as an antibacterial lead with Gram-negative selectivity.
Journal: Nature Chemical Biology - NAT CHEM BIOL , vol. 5, no. 11, pp. 849-856, 2009
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
Sort by:

Citations (9)

Share this on