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Multilocus sequence analysis of the genus Bradyrhizobium

Multilocus sequence analysis of the genus Bradyrhizobium,10.1016/j.syapm.2008.12.005,Systematic and Applied Microbiology,Raul Rivas,Miet Martens,Phili

Multilocus sequence analysis of the genus Bradyrhizobium   (Citations: 11)
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The use of multilocus sequence analysis (MLSA) for the taxonomy of Bradyrhizobium was assessed. We compared partial sequences for atpD, recA, gyrB, rpoB and dnaK for a set of reference strains representing named species and genospecies, and a number of new isolates from Lupinus albus, Arachis hypogaea and Ornithopus compressus from Spain. The phylogenies of the individual genes were compared with previous DNA–DNA hybridization results. High hybridization values were well reflected, but intermediary hybridization values were less clearly apparent. However, the phylogeny of a concatenated dataset of the five genes did reflect all values and thus is more informative of overall genome similarity. Our results indicate that only for the genes gyrB, rpoB and dnaK there is a small gap between the interspecies sequence similarities and the intraspecies similarity, and therefore cut-off levels for species delineation cannot be set, although high sequence similarity (>99%) does permit identification. In a few instances, a reference strain did not group as expected for one of the five genes tested. This may be a result of horizontal gene transfer and recombination events occasionally involving housekeeping genes. This observation indicates it is best to consider more than one gene for taxonomic inferences. The majority of the new isolates from the three host species was identified as Bradyrhizobium canariense. Four strains from L. albus from León, Spain, formed a separate group close to Bradyrhizobium japonicum.
Journal: Systematic and Applied Microbiology - SYST APPL MICROBIOL , vol. 32, no. 2, pp. 101-110, 2009
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    • ..., 2010) and Bradyrhizobium canariense BTA-T (...

    Martha E Trujilloet al. The genus Micromonospora is widespread in legume root nodules: the exa...

    • ...The strains isolated to date from effective nodules of L. albus in different countries belong to the genus Bradyrhizobium (Barrera et al. 1997 ;J arabo-Lorenzo et al. 2003; Stepkowski et al. 2007; Rivas et al. 2009)...
    • ...Several housekeeping genes have been analysed for the strains isolated in mainland Spain (Stepkowski et al. 2007; Rivas et al. 2009 )b ut therrs gene and the 16S-23S intergenic spacer (ITS) have not been previously analysed...
    • ...RLA08 Le ´on (Spain) L. albus Rivas et al. (2009) F III A RLA09 Le ´on (Spain) L. albus Rivas et al. (2009) G III A...
    • ...RLA08 Le ´on (Spain) L. albus Rivas et al. (2009) F III A RLA09 Le ´on (Spain) L. albus Rivas et al. (2009) G III A...
    • ...RLA10 Le ´on (Spain) L. albus Rivas et al. (2009) H III A...
    • ...RLA11 Le ´on (Spain) L. albus Rivas et al. (2009) I III A...
    • ...MCLA07 Salamanca (Spain) L. albus Rivas et al. (2009) J I* D MCLA12 Salamanca (Spain) L. albus Rivas et al. (2009) K II* D...
    • ...MCLA07 Salamanca (Spain) L. albus Rivas et al. (2009) J I* D MCLA12 Salamanca (Spain) L. albus Rivas et al. (2009) K II* D...
    • ...MCLA22 Salamanca (Spain) L. albus Rivas et al. (2009) L I* C...
    • ...In recent years, analyses of housekeeping genes have been performed in Bradyrhizobium showing their usefulness in taxonomic and phylogenetic studies (Vinuesa et al. 2005a and b; Stepkowski et al. 2007; Ramı´rez-Bahena et al. 2009; Rivas et al. 2009)...
    • ...From these genes, the atpD gene has been previously analysed in strains from Lupinus isolated in different geographical locations (Stepkowski et al. 2007; Rivas et al. 2009)...
    • ...In these previous studies, some strains isolated from L. albus in Spain and the Canary Islands were already included, with the conclusion that they belong to different phylogenetic groups (Stepkowski et al. 2007; Rivas et al. 2009)...

    Encarna Velázquezet al. Strains nodulating Lupinus albus on different continents belong to sev...

    • ...Turner and Young 2000; Gaunt et al. 2001; Alexandre et al. 2008; Martens et al. 2008; Vinuesa et al. 2008; Ribeiro et al. 2009; Rivas et al. 2009; Menna et al. 2009)...

    Ilmara Varotto Roma Netoet al. Genetic diversity of elite rhizobial strains of subtropical and tropic...

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