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Complete mitochondrial genome phylogeographic analysis of killer whales (Orcinus orca) indicates multiple species

Complete mitochondrial genome phylogeographic analysis of killer whales (Orcinus orca) indicates multiple species,10.1101/gr.102954.109,Genome Researc

Complete mitochondrial genome phylogeographic analysis of killer whales (Orcinus orca) indicates multiple species   (Citations: 21)
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P. A. Morin, F. I. Archer, A. D. Foote, J. Vilstrup, E. E. Allen, P. Wade, J. Durban, K. Parsons, R. Pitman, L. Li, P. Bouffard, S. C. Abel Nielsenhttp://academic.research.microsoft.com/io.ashx?type=5&id=37806127&selfId1=0&selfId2=0&maxNumber=12&query=
Journal: Genome Research - GENOME RES , vol. 20, no. 7, pp. 908-916, 2010
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    • ...), where resource partitioning and foraging specializations of sympatric populations occurring in the North Pacific have lead to the evolution of distinct lineages ...

    Ana R. Amaralet al. Seascape Genetics of a Globally Distributed, Highly Mobile Marine Mamm...

    • ... Sequence tags enable efficient distribution of the output from these platforms among many individually identifiable samples rather than extensive, deep sequencing of single individuals or mixed samples. Thus, the ability to tag and track sequenced DNA from many individuals in multiplex increases the efficiency of MPS when the genomes being sequenced are small ...

    Brant C. Fairclothet al. Not All Sequence Tags Are Created Equal: Designing and Validating Sequ...

    • ...). Uncertainty in body size should be incorporated in ecosystem models, especially given recent evidence for multiple killer whale species worldwide ...

    Rob Williamset al. Competing Conservation Objectives for Predators and Prey: Estimating K...

    • ...The use of high-throughput sequencing and pooled tagging methods [16] has the potential to generate large amounts of manifold-coverage sequence data for large numbers of samples quickly and at a relatively low cost, allowing improved resolution of phylogenetic relationships to be achieved routinely (e.g., [17])...
    • ...This is the first time complete mitogenome sequences have been published for five of the species, and mitogenome sequences from a further three species have previously only been used as outgroup species for an intra-specific phylogenetic study on the killer whale [17]...
    • ...[17] Globicephala macrorhynchus* Short-finned pilot whale JF339975 This study Globicephala macrorhynchus* Short-finned pilot whale JF339976 This study Globicephala macrorhynchus Short-finned pilot whale JF339974 This study Globicephala melas* Long-finned pilot whale JF339973 This study Globicephala melas Long-finned pilot whale HM060333 Ref...
    • ...[17] Globicephala melas Long-finned pilot whale JF339972 This study Pseudorca crassidens False killer whale JF289173 This study Pseudorca crassidens False killer whale JF289174 This study Pseudorca crassidens False killer whale HM060332 Ref...
    • ...[17] Grampus griseus Risso’s dolphin EU557095 Ref...
    • ...[17] Orcinus orca Killer whale GU187180 Ref...
    • ...[17] Steno bredanensis* Rough-toothed dolphin JF339982 This study Stenella attenuata Pantropical spotted dolphin EU557096 Ref...
    • ...Increased sampling and mitogenome sequencing, such as conducted on the killer whale [17], is needed to confirm complete lineage sorting for the remaining species included in this study...
    • ...The mitochondrial genome was amplified in 3-5 overlapping amplicons (dependent on DNA preservation) using previously published long-range PCR primers [17] and primers designed specifically for this study (see Additional file 3). Additional sequencing primers were also designed for gap filling using conventional Sanger sequencing at the commercial service offered by Macrogen (Seoul, South Korea)...
    • ...A total of 35 mitogenome sequences were used in the analyses, representing 21 species (Table 1). Of these, 18 mitogenomes were amplified and sequenced for this study and 17 mitogenomes attained from Genbank [17,30,48,49], which included 4 outgroup sequences (narwhal, Monodon monoceros; harbor porpoise, Phocoena phocoena; Yangtze river dolphin, Lipotes vexillifer; Amazon river dolphin, Inia geoffrensis)...

    Julia T Vilstrupet al. Mitogenomic phylogenetic analyses of the Delphinidae with an emphasis ...

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