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Molecular cloning and biochemical characterization of a lipoxygenase in almond (Prunus dulcis) seed

Molecular cloning and biochemical characterization of a lipoxygenase in almond (Prunus dulcis) seed,10.1046/j.1432-1327.2001.02020.x,European Journal

Molecular cloning and biochemical characterization of a lipoxygenase in almond (Prunus dulcis) seed   (Citations: 17)
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Journal: European Journal of Biochemistry - EUR J BIOCHEM , vol. 268, no. 5, pp. 1500-1507, 2001
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    • ...Some LOXs, such as r9-LOX1 and OsLOX1 in rice [10,11] and PdLOX in almond [12], were found to be involved in germination...
    • ...Other LOXs from group II, such as ZmLOX3 [7,33], OsLOX1 [11], and PdLOX [12], are reported as being involved in the defence mechanism, whereas olive LOX is reported to be involved in fruit ripening and senescence [8]...

    Shouan Liuet al. Differential expression pattern of an acidic 9/13-lipoxygenase in flow...

    • ...In almond, a LOX gene involved in early seed development was characterized with maximal transcript levels and enzyme activity about 30 d after flowering and then declining (Mital et al., 2001), and a LOX gene from hazelnut has been associated with early seed development (Santinol et al., 2003)...

    Bo Zhanget al. Differential expression within the LOX gene family in ripening kiwifru...

    • ...Scorza Verde) genomic DNA was extracted from young seedlings, and used for the construction of a genomic library as described previously (Mita et al., 2001)...
    • ...HPL activity during almond seed development It has been shown previously that 9-LOXs are expressed and active at early developmental stages of almond seeds before lipid body deposition begins (Mita et al., 2001)...
    • ...Cloning and characterization of a putative almond HPL gene An almond genomic library was prepared as described (Mita et al., 2001), and a positive clone was identified using a 9-HPL-encoding cDNA from cucumber (accession number AF229811; Matsui et al., 2000) as a probe...
    • ...9-HPL is present throughout seed development, but its substrate may be available only at the early stages of seed development because 9-LOX activity is limited to these stages (Mita et al., 2001; Santino et al., 2005); its products should, however, be readily available to 9-HPL which is localized to microsomes and on the surface of lipid bodies...

    Giovanni Mitaet al. Molecular cloning and characterization of an almond 9-hydroperoxide ly...

    • ...The intentions have been variable: in some cases to simply understand the nature of enzyme specificity (Royo et al, 1996), in others to establish a role in the aroma of foods (Mita et al, 2001), and in others still to produce LOX in sufficient amounts (gram quantities) for industrial use (Hughes et al, 1999)...

    Rod Caseyet al. Recombinant Lipoxygenases and Oxylipin Metabolism in Relation to Food ...

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