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Functional roles of the pre-sensor I insertion sequence in an AAA+ bacterial enhancer binding protein

Functional roles of the pre-sensor I insertion sequence in an AAA+ bacterial enhancer binding protein,10.1111/j.1365-2958.2009.06744.x,Molecular Micro

Functional roles of the pre-sensor I insertion sequence in an AAA+ bacterial enhancer binding protein   (Citations: 7)
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Journal: Molecular Microbiology - MOL MICROBIOL , vol. 73, no. 4, pp. 519-533, 2009
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    • ...7 ml min−1 flow rate using a BioCad Sprint HPLC system and a Bio-Sep-S 3000 column (Phenomenex), as described previousl...

    Milija Jovanovicet al. Regulation of the co-evolved HrpR and HrpS AAA+ proteins required for ...

    • ...bEBPs are classified within Clade 6 of the AAA+ protein family (10) and are distinguished from other subclasses of AAA+ proteins by two sequence insertions: the L1 loop containing the s54-interacting GAFTGA motif and the L2 loop (also termed the pre sensor I insertion loop, pre-SIi loop) thought to co-ordinate movement of the L1 loop (11)...

    Nan Zhanget al. The role of the conserved phenylalanine in the p54-interacting GAFTGA ...

    • ...(located at the C-terminus of Helix 4) has been proposed to contact and co-ordinate the movements of the L1 loop (20)...
    • ... 7% and 14% of the intensity of the ADP–AlF complex), compared to either the ADP–AlF or AMP–AlF (which also exhibits reduced Native-PAGE stability of between 44% and 85% of the intensity of the ADP–AlF complex) complexes, may represent an altered arrangement or stabilization of the L1 and L2 loops (within the PspF1–275:ADP–BeF complex) with respect to the activator interacting surfaces on s54, independent of the s54–DNA interactions (20)...
    • ...Engagement of the Es54 closed complex by PspF (bEBP) requires the appropriate presentation of the surface exposed (s54 interacting) L1 loop, which is determined by the nucleotide-bound state of PspF and also probably depends upon favourable interactions between the L1 and L2 loops (20)...

    Patricia C. Burrowset al. Comparative analysis of activator-Ep54 complexes formed with nucleotid...

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