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Purification and characterization of pepsins A1 and A2 from the Antarctic rock cod Trematomus bernacchii

Purification and characterization of pepsins A1 and A2 from the Antarctic rock cod Trematomus bernacchii,10.1111/j.1742-4658.2007.06136.x,Febs Journal

Purification and characterization of pepsins A1 and A2 from the Antarctic rock cod Trematomus bernacchii   (Citations: 7)
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Journal: Febs Journal - FEBS J , vol. 274, no. 23, pp. 6152-6166, 2007
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    • ...In the past decades, studies on PGs and pepsins from fish such as rainbow trout (Oncorhynchus mykiss; Twining et al. 1983), North Pacific bluefin tuna (Thunnus thynuus orientalis; Tanji et al. 1988, 1996), Atlantic cod (Gadus morhua; Gildberg et al. 1990) and shark (Centroscymnus coelolepsis; Nguyen et al. 1998), Antarctic rock cod (Trematomus bernacchii; Brier et al. 2007), Africa coelacanth (Latimeria chalumnae; Tanji et al. 2007), ...
    • ...Similar optimal temperature of pepsin 1 and pepsin 2 profiles was documented in pepsins from smooth hound (Bougatef et al. 2008), pectoral rattail (Klomklao et al. 2007), pepsin 2, and pepsin 3 from snakehead (Chen et al. 2009), while pepsins from Antarctic rock cod (Brier et al. 2007) and orange roughy (Hoplostethus atlanticus; Xu et al. 1996) was similar to pepsin 3. Furthermore, similar to smooth hound pepsin, the activity of these ...

    Wu-Yin WengTaoet al. Purification and characterization of pepsinogens and pepsins from the ...

    • ...The enzymes were purified, and their biochemical properties were studied in comparison to pepsin A from porcine stomach (Brier et al., 2007)...
    • ...To determine the purity and validate the correct mass of the purified fish pepsinogens, ESI MS was used (Brier et al., 2007)...
    • ...Both fish pepsin A1 and fish pepsin A2 were less efficient at cleaving peptide 1 than was pig pepsin (Brier et al., 2007)...
    • ...The peptides in each part of the HPLC separation were visualized, compared, and then sequenced by tandem MS (MS⁄MS) (Brier et al., 2007)...
    • ...Further, pig pepsin produced many fragments in one portion of the substrate protein, whereas the fish pepsins preferred other regions (Brier et al., 2007)...
    • ...The two Antarctic isoenzymes are, to our knowledge, the first Antarctic fish pepsinogens obtained using the strategy of expression in E. coli followed by refolding and purification (Brier et al., 2007)...

    Viviana De Lucaet al. Aspartic proteinases in Antarctic fish

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