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Molecular cloning of Kunitz-type proteinase inhibitor group B genes from potato

Molecular cloning of Kunitz-type proteinase inhibitor group B genes from potato,10.1007/s10541-005-0114-3,Biochemistry-moscow,A. S. Speranskaya,A. A.

Molecular cloning of Kunitz-type proteinase inhibitor group B genes from potato   (Citations: 3)
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Eighteen clones representing copies of four Kunitz-type proteinase inhibitor group B genes (PKPI-B) obtained by polymerase chain reaction cloning of potato (Solanum tuberosum L. cv. Istrinskii) genomic DNA were sequenced and analyzed. Three new genes were found and named PKPI-B1, PKPI-B2, and PKPI-B10: these were represented by five, two, and seven clones, respectively. The remaining four clones corresponded to the formerly characterized PKPI-B9 gene. These data show that at least four PKPI-B encoding genes are harbored in the genome of potato cv. Istrinskii. Their analysis suggests that variability of PKPI-B encoding genes in potato is limited and could be explained by cross-hybridization events in the ancestor forms rather than by random mutagenesis.
Journal: Biochemistry-moscow - BIOCHEMISTRY-ENGL TR , vol. 70, no. 3, pp. 292-299, 2005
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