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Chromosome banding and FISH with rDNA probe in the diploid and tetraploid loach Misgurnus anguillicaudatus

Chromosome banding and FISH with rDNA probe in the diploid and tetraploid loach Misgurnus anguillicaudatus,10.1007/s10228-010-0168-0,Ichthyological Re

Chromosome banding and FISH with rDNA probe in the diploid and tetraploid loach Misgurnus anguillicaudatus   (Citations: 1)
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The chromosomes of the diploid and tetraploid loach Misgurnus anguillicaudatus were analyzed by staining with Ag, chromomycin A3 (CMA3)/distamycin A (DA), and DA/4′,6-diamidino-2-phenylindole (DAPI), and using fluorescence in situ hybridization (FISH) with 5.8S + 28S rDNA as a probe. Nucleolus organizer regions (NORs) were mapped to the telomeric region of the short arms of the largest (first) metacentric chromosome pair in the diploid loach with 2n = 50 and the homologous quartet in the tetraploid loach with 4n = 100. The NORs were positive at the same region of the first metacentric chromosome for Ag and CMA3/DA stainings, but negative for DA/DAPI staining. Four signals at the homologs within the same quartet suggest the duplication of the entire genome from diploid to tetraploid status. However, a size difference was detected between the rDNA signals by FISH and CMA3 banding.
Journal: Ichthyological Research - ICHTHYOL RES , vol. 57, no. 4, pp. 358-366, 2010
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    • ...The chromosomes bearing nucleolar organizing regions (NORs) were used as a marker to detail the pairing profile of homologous chromosomes, after differential fluorochrome staining and fluorescence in situ hybridization (FISH) using a 5.8S ? 28S rDNA probe (Li et al. 2010)...
    • ...FISH with human 5.8S ? 28S rDNA as a probe (Fujiwara et al. 1998), after labeling with biotin-16-dUTP by nick translation (Roche), was conducted following Li et al. (2010)...
    • ...Hybridization signals were photographed under a Nikon Eclipse E800 fluorescence microscope as previously (Li et al. 2010)...
    • ...(Figs. 1a, 2a), confirming the diploid chromosome number of 50 (Li et al. 2010)...
    • ...This suggests a tail-to-tail association of two homologous chromosomes because the CMA3-positive site is located at the telomeric region of the short arms of the largest metacentric chromosomes (Li et al. 2010)...

    Ya-Juan LiZhuoet al. The origin of natural tetraploid loach Misgurnus anguillicaudatus (Tel...

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