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Protein complexed with chondroitin sulfate in poly(lactide- co-glycolide) microspheres

Protein complexed with chondroitin sulfate in poly(lactide- co-glycolide) microspheres,10.1016/j.biomaterials.2007.01.049,Biomaterials,Eun Seong Lee,K

Protein complexed with chondroitin sulfate in poly(lactide- co-glycolide) microspheres   (Citations: 11)
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Chondroitin sulfate (CsA) is an acidic mucopolysaccharide, which is able to form ionic complexes with positively charged proteins. In this study, a protein–CsA complex was constructed to nano-sized particles. Zeta potential measurements revealed that a CsA-to-protein fraction of greater than 0.1 results in a neutralization of the positive charge on lysozyme (Lys). Based on this preliminary study, we have prepared poly(lactide-co-glycolide) (PLGA) microspheres harboring Lys/CsA complexes via the multi-emulsion method. Protein stability in the PLGA microspheres was preserved during both microsphere preparation and protein release. The profiles of Lys release from the PLGA microspheres evidenced nearly zero-order kinetics, depending on the quantity of CsA. An in vivo fluorescent image of experimental mouse tissue showed that the PLGA microspheres with the Lys/CsA complex had released the entirety of their Lys without no residual amount after 23 days, but microspheres without the complex harbored a great deal of residual Lys, which is attributable to its degradation by acidic PLGA degradates. The tissue reaction evidenced by the PLGA microspheres stabilized with CsA showed minimal foreign body reaction and little configuration of immune cells including neutrophils and macrophages, but the reactions of the PLGA microspheres without CsA were characterized by a relatively elevated inflammation. These results show that CsA is a viable candidate for long-acting micro-particular protein delivery.
Journal: Biomaterials , vol. 28, no. 17, pp. 2754-2762, 2007
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    • ...Encapsulation of complexes of cationic lysozyme with variable amounts of anionic polyelectrolyte chondroitin sulfate (CsA) by a W/O/W method resulted in microspheres with enhanced encapsulation efficiency compared to native lysozyme, with simultaneous reduction in the amount of insoluble lysozyme aggregates (56)...

    Sheetal S. Paiet al. Poly(ethylene glycol)Modified Proteins: Implications for Poly(lactide-...

    • ...Recently, glycosaminoglycan (GAG) family for stabilization in the protein loading PLGA depot was focused by our group [17]...
    • ...The DS/BSA complex may enhance protein stability in organic/water interface during preparation procedure of PLGA depot, because numerous sulfate groups on DS mediate, a hyper-structural shielding of protein with positively charge via ionic interacts at pH 3.0 [17]...
    • ...The actual protein loading efficiency was determined using the following equations [17]...
    • ...In many cases, proteins ionic complex have been encapsulated within biodegradable polymer [17,33-35]...

    Kun Na. Dermatan sulfate as a stabilizer for protein stability in poly(lactide...

    • ...The complex formation of the protein drugs with the oppositely charged polymer has shown to improve protein stability in the microsphere (Lee et al., 2007b)...

    Seong Ju Naet al. Stability and bioactivity of nanocomplex of TNF-related apoptosis-indu...

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