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In vitro comparison of egg yolk–based and soybean lecithin–based extenders for cryopreservation of ram semen

In vitro comparison of egg yolk–based and soybean lecithin–based extenders for cryopreservation of ram semen,10.1016/j.theriogenology.2009.10.005,Ther

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In vitro comparison of egg yolk–based and soybean lecithin–based extenders for cryopreservation of ram semen   (Citations: 4)
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Substitution of egg yolk with soybean lecithin may reduce hygienic risks in extenders. Though a few studies have been performed on the effect of soybean lecithin in bull, to date evaluation of ram semen in vitro fertility after cryopreservation with use of soybean lecithin has not been studied. This study assessed the effect of 1% or 2% (wt/vol) soybean lecithin (L1 or L2) or 15% or 20% (vol/vol) egg yolk (E15 or E20) supplemented with 5% or 7% glycerol (G5 or G7) in a Tris-based medium for cryopreservation of ram (Oviss arries) semen. Although no significant difference was observed in pattern of capacitation, the best results in terms of sperm motility, viability postthaw, and cleavage rates were observed with L1G7 (51.9±4.8%, 48.1±3.5%, and 79.6±3.9%, respectively) and E20G7 (51.8±2.9%, 46.7±4.0%, and 72.9±6.4%, respectively). Our results also showed that 1% lecithin and 20% egg yolk was superior to 2% lecithin and 15% egg yolk. In terms of cleavage rate, 7% glycerol was superior to 5% glycerol. No significant difference was obtained between groups in terms of blastocysts rate per cleaved embryo. Therefore, we concluded that the optimal concentration of lecithin and egg yolk is 1% and 20%, respectively, along with 7% glycerol. In addition, our results suggest that lecithin can be used as a substitute for egg yolk.
Journal: Theriogenology , vol. 73, no. 4, pp. 480-487, 2010
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    • ...We previously reported, for the first time, that 1% lecithin can replace egg yolks for ram sperm cryopreservation (20)...
    • ...The basic extender used in this study was prepared according to our previous publication (20)...
    • ...Semen parameters, the hypo-osmotic swelling test (HOST) and chlortetracycline (CTC) assay were carried out according to Forouzanfar et al. (20), Revell and Marode (21) and Perez et al. (22), respectively...
    • ...In vitro fertilization (IVF) Oocyte recovery and in vitro maturation This procedure was carried out according to Forouzanfar et al. (20)...
    • ...Considering the aforementioned fact, in addition to inconsistency in the individual quality differences that are inherent in egg yolks (7, 14, 26), it is apparent that substitution of egg yolk with animal free products would have several advantages including enhancing consistency in the components of semen extenders and exclusion of hygienic risks (7, 14, 16- 20)...
    • ...The results of our previous study revealed that the substitution of egg yolk with 1% lecithin resulted in development of a suitable extender for cryopreservation of ram semen (20)...

    Mohsen Sharafi, et al. In Vitro Comparison of Soybean Lecithin Based-Extender with Commercial...

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