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Isolation and identification of bacteriophages infecting ayu Plecoglossus altivelis altivelis specific Flavobacterium psychrophilum

Isolation and identification of bacteriophages infecting ayu Plecoglossus altivelis altivelis specific Flavobacterium psychrophilum,10.1016/j.vetmic.2

Isolation and identification of bacteriophages infecting ayu Plecoglossus altivelis altivelis specific Flavobacterium psychrophilum   (Citations: 2)
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In order to investigate methods for controlling systemic bacterial coldwater disease (CWD), bacteriophages that infect Flavobacterium psychrophilum were isolated by the enrichment method from pond water collected from Japanese ayu farms. The five phages isolated were classified as members of Myoviridae (PFpW-3, PFpC-Y), Podoviridae (PFpW-6, PFpW-7), and Siphoviridae (PFpW-8) and had highly variable patterns of infectivity for different F. psychrophilum isolates (n=128). The stability tests of the phages in different waters, pHs and temperatures were assessed, and the results indicated that none of the phages were affected by ayu farm conditions. Among the phages, PFpW-3 had high infectivity for F. psychrophilum isolated from ayu and other fish and demonstrated sufficient survivability in the stability tests. Thus, PFpW-3 and its indicator strain N2-3 were inoculated into cytophaga broth at different doses of multiplicity of infection (MOI) and proved to be efficient for the reduction of bacterial growth. This study may be the basis for a further evaluation of phage therapy in the treatment of CWD in Japanese ayu farms.
Journal: Veterinary Microbiology - VET MICROBIOL , vol. 140, no. 1, pp. 109-115, 2010
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    • ...Bacteriophage therapy has been widely attempted to treat different animal diseases induced by different types of bacteria (Smith and Huggins 1982, 1983; Barrow et al. 1998; Park et al. 2000; Sheng et al. 2006; Jamalludeen et al. 2007; Stenholm et al. 2008; Kim et al. 2010; Oliveira et al. 2010)...

    Hao Liet al. Biosafety evaluation of bacteriophages for treatment of diarrhea due t...

    • ...et al. 2003; Buiser et al. 2009; Faquet et al. 2005; Governal and Gerba 1997; Goulet et al. 2010; ICTVdB Management 2006; McAuliffe et al. 2007; Kivelä 2004; Kumari et al. 2010; Kim et al. 2010; Lin et al. 2010; Prangishvili et al. 2001; Pringsulaka et al. 2010; Tey et al. 2009; Wachman and Brown 2010; Wilson et al. 2004 Table 1 (continued) Folia Microbiol...
    • ...The stability of five Flavobacterium phages (PFpW-3, PFpC-Y, PFpW-6, PFpW-7, PFpW-8) isolated from pond water collected from Japanese ayu farms was tested for 21 days at 18°C (Kim et al. 2010)...

    E. Jończyket al. The influence of external factors on bacteriophages—review

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