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Changes in levels, species and DNA fingerprints of soil microorganisms associated with cotton expressing the Bacillus thuringiensis var. kurstaki endotoxin

Changes in levels, species and DNA fingerprints of soil microorganisms associated with cotton expressing the Bacillus thuringiensis var. kurstaki endo

Changes in levels, species and DNA fingerprints of soil microorganisms associated with cotton expressing the Bacillus thuringiensis var. kurstaki endotoxin   (Citations: 113)
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An important aspect of the risk assessment of pesticidal transgenic plants is the potential for detrimental effects on the soil ecosystem from residual plant material following harvesting and tillage. We evaluated this concern by placing leaves of three different lines of cotton genetically engineered to produce the Bacillus thuringiensis var. kurstaki (B.t.k.) endotoxin in soil and monitoring numbers and species of indigenous soil bacteria and fungi. Four experiments, lasting 28 or 56 days, were performed using combinations of the following treatments: (1) soil only; (2) soil +purified B.t.k. toxin; (3) soil +parental cotton; (4) soil +purified B.t.k. toxin +parental cotton; (5) soil + B.t.k. toxin-producing cotton.Two of the three transgenic cotton lines caused a transient increase in total bacterial and fungal population levels that was significantly higher on several sample days in the experiments than the levels in the other treatments. In contrast, neither the third transgenic cotton line nor the purified B.t.k. toxins had any significant effects on the total numbers of bacteria and fungi.Transient changes in bacterial species composition, measured by biochemical tests of individual cultures, community substrate utilization and DNA fingerprinting, were also observed in treatments with the two transgenic plant lines.The plant line specificity of the response, and the lack of effects from the purified B.t.k. toxins, suggest that the observed effects of the two transgenic plant lines on soil microorganisms may not have resulted from the plants' production of B.t.k. toxin. We suggest that genetic manipulation or tissue culturing of the plants may have produced a change in plant characteristics, aside from B.t.k. toxin production, that can influence growth and species composition of soil microorganisms.
Journal: Applied Soil Ecology - APPL SOIL ECOL , vol. 2, no. 2, pp. 111-124, 1995
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    • ...For example, Donegan, Palm, and Fieland (1995) reported a transient stimulation of soil bacterial and fungal populations by two of three Bt cotton lines...

    Wude Yanget al. Effect of Transgenic Bt Cotton on Bioactivities and Nutrients in Rhizo...

    • ...1995) or a decrease in their diversity...

    Anu Kaliaet al. Effect of pesticide application on soil microorganisms

    • ...Donegan et al. (1995) found significant differences in population size, species and composition of microbiota in soil associated with two varieties of Bt cotton and their parental cotton using DNA fingerprinting method (Donegan et al. 1995)...
    • ...Donegan et al. (1995) found significant differences in population size, species and composition of microbiota in soil associated with two varieties of Bt cotton and their parental cotton using DNA fingerprinting method (Donegan et al. 1995)...
    • ...It has been widely reported that transgenic proteins such as Cry1Ab can be released into the soil by means of cotton residuals, root exudates, and pollen in the course of growth and after harvest (Donegan et al. 1995; Gupta and Watson 2004; Knox et al. 2007; Palm et al. 1996; Rui et al. 2005)...

    Xiaogang Liet al. No evidence of persistent effects of continuously planted transgenic i...

    • ...Changes in the total numbers and species composition of culturable aerobic bacteria are also determined by using the plating method (Donegan et al. 1995)...
    • ...Likewise, transient but significant changes in soil bacterial species composition, measured by biochemical assays of individual cultures, have been observed in two out of three lines of cotton genetically engineered to produce the Bacillus thuringiensis var. kurstaki endotoxin (Donegan et al. 1995)...
    • ...In some studies, differences in microbial characteristics between soils planted with transgenic or non-transgenic plants have been found (Donegan et al. 1995; Siciliano et al. 1998; Sessitsch et al. 2002)...

    Natália Faragováet al. Effect of transgenic alfalfa plants with introduced gene for Alfalfa M...

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