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Isolation and partial characterization of pepsin-soluble collagen from the skin of grass carp ( Ctenopharyngodon idella)

Isolation and partial characterization of pepsin-soluble collagen from the skin of grass carp ( Ctenopharyngodon idella),10.1016/j.foodchem.2006.09.05

Isolation and partial characterization of pepsin-soluble collagen from the skin of grass carp ( Ctenopharyngodon idella)   (Citations: 21)
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Pepsin-soluble collagen was extracted from the skin of grass carp (Ctenopharyngodon idella) with a yield of 46.6%, on a dry weight basis. Electrophoretic patterns showed that the collagen contained α1 and α2 chains, similar to those of calf skin collagen. The imino acid content of the collagen from grass carp skin was much lower than those of mammalian’s collagens, as also were the transition temperature and denaturation temperature which were only 24.6°C and 28.4°C respectively. Peptide maps of the collagen digested by trypsin and V8 protease showed different peptide fragments from those of calf skin collagen and other fish skin collagens, suggesting differences in amino acid sequences and collagen conformation. In addition, the lyophilized collagen sponge from grass carp skin had a uniform and regular network structure, just like calf skin collagen sponge. These results suggest that grass carp skin has potential for use as a supplementary source of collagen.
Journal: Food Chemistry - FOOD CHEM , vol. 103, no. 3, pp. 906-912, 2007
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    • ...The denaturation temperature was measured from the viscosity change, using an NDJ-8S viscosimeter (Zhengji Instruments Co, Ltd, Jiangsu province, China), according to the modified method of Zhang et al. (2007)...

    Wandong Fuet al. Isolation and Characterization of Pepsin-Soluble Collagen from the Ski...

    • ...The molecular structure of collagen is maintained mainly by restrictions on changes in the secondary structures of the polypeptide chain, imposed by the pyrrolidine rings of proline and hydroxyproline (Zhang et al, 2007)...

    Shanshan Wanget al. Characterization of Acid-Soluble Collagen from Bone of Pacific Cod (Ga...

    • ...Grass carp Actinopterygii Cyprinidae Ctenopharyngodon idella Skin 42.04 15.65 0.41 3.1 273 87 1 Zhang et al. 2007...
    • ...flounder Actinopterygii Paralichthyidae Paralichthys olivaceus Muscle; skin 41.30 15.54 0.41 3.1 269 87 2 Nishimoto et al. 2005 Bigeye snapper Actinopterygii Pricanthidae Priacanthus tayenus Skin; bone 42.24 15.49 0.35 3.2 337 106 5 Jongjareonrak et al. 2005; Kittiphattanabawon et al. 2005; Zhang et al. 2007...
    • ...puffer Actinopterygii Tetraodontidae Takifugu rubipes Skin; muscle 41.41 15.49 0.44 3.1 254 81 4 Nagai et al. 2002; Yata et al. 2001; Zhang et al. 2007...
    • ...Cattle Mammalia Bovidae Bos taurus Bone; skin; muscle 42.32 15.28 0.18 3.2 643 199 5 Eastoe 1955; Eastoe and Leach 1958; Kawaguchi 1993; Li et al. 2004; Zhang et al. 2007...
    • ...Pig Mammalia Suidae Sus scrofa Muscle; skin 42.53 15.26 0.18 3.3 660 203 3 Katafuchi et al. 2007; Zhang et al. 2007...

    Olaf Nehlichet al. Establishing collagen quality criteria for sulphur isotope analysis of...

    • ...The denaturation temperature was measured from changes in viscosity, using an Ubbelohde viscometer, according to the method modified from Zhang et al. (2007)...
    • ...The content of ASC fraction in squid skin was different from those reported in cuttlefish skin (2.0%) (Nagai et al., 2001), ocellate puffer fish skin (10.7%) (Nagai et al., 2002), and grass carp skin (8%) (Zhang et al., 2007)...
    • ...The imino acid (proline and hydroxyproline) contents of ASC and PSC from squid skin were 18.0% and 18.7%, respectively, close to that of 18.6% in grass carp collagen (Zhang et al., 2007), but lower than that of 22% in porcine skin collagen (Ikoma et al., 2003) and 21.5% in bovine skin collagen (Cui et al., 2007)...
    • ...lock (Yan et al., 2008), grass carp, bovine (Zhang et al., 2007) and porcine, indicating that α chains of squid skin collagen were distinct in its primary structure...
    • ...Td of squid skin collagen was 29.4℃, close to those of collagens from skins of ocellate puffer fish (28.0℃) (Nagai et al., 2002), grass carp (28.4℃) (Zhang et al., 2007), and brown backed toadfish (28.0℃) (Senaratne et al., 2006), higher than that of cod skin collagen (15.0℃) (Rigby, 1968), but about 8℃ lower than that of collagen from porcine skin (37.0℃) (Nagai et al., 1999)...
    • ...ondary structure of the polypeptide chain (imposed by the pyrrolidine rings of proline and hydroxyproline), and also maintained partially by the hydrogen bond ability through the hydroxy group of hydroxyproline (Zhang et al., 2007)...

    Mingyan Yanet al. Isolation and characterization of collagen from squid ( Ommastrephes b...

    • ...Pepsin solubilized collagen was extracted from the skin of grass carp (Ctenopharyngodon idella) with a yield of 35% (dry weight basis) (Zhang et al., 2007)...

    Sappasith Klomklao. Digestive proteinases from marine organisms and their applications

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