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Site-specific effects of 17β-estradiol in hornyhead turbot ( Pleuronichthys verticalis) collected from a wastewater outfall and reference location

Site-specific effects of 17β-estradiol in hornyhead turbot ( Pleuronichthys verticalis) collected from a wastewater outfall and reference location,10.

Site-specific effects of 17β-estradiol in hornyhead turbot ( Pleuronichthys verticalis) collected from a wastewater outfall and reference location   (Citations: 1)
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Studies throughout the southern California bight have indicated persistent estrogenic activity in male hornyhead turbot (Pleuronichthys verticalis). Plasma 17β-estradiol (E2) concentrations correlated with gonadal DNA damage in fish collected near a wastewater treatment plant outfall, but not from fish collected at the reference location. When the same species was collected from the same reference location and treated with E2, no relationship between uptake and gonadal DNA damage was observed. To evaluate the site-specific effects of E2 in fish from a wastewater outfall and fish from a reference location, male hornyhead turbot from each location were exposed to 15μg/L aqueous E2 in a time-course experiment, with fish sampled every 12h for 48h. Concentrations of E2 were measured in the aqueous exposure and in plasma from the fish. Vitellogenin (vtg) was also measured in the plasma, and 8-oxo-7,8-dihydro-2′-deoxyguanosine levels in male gonads were measured as an indicator of DNA damage. Untreated fish from the outfall had significantly lower E2 in the plasma relative to the untreated reference fish, and this trend was consistent at each time point in the E2-treated fish. Vtg was significantly induced after 36h of exposure in fish from both sites and no significant differences were observed between the sites. A significant increase of oxidative DNA damage was observed in E2-treated fish from the outfall population and the damage was significantly correlated with plasma E2 concentrations only in fish from the outfall after 48h. These results indicated that there were significant differences in E2 disposition and gonadal genotoxicity between the hornyhead turbot populations following exposure to E2, suggesting that fish at wastewater outfalls may be more sensitive to DNA damage, which may be temporally related to concentrations of E2 in plasma.
Journal: Environmental Research - ENVIRON RES , vol. 109, no. 5, pp. 552-558, 2009
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    • ...Elevated levels of plasma E2 concentration leading to DNA damage in fish are reported (Rempel-Hester et al. 2009)...
    • ...Studies in mammals show that estrogens are linked with oxidative DNA damage and DNA adduct formation, probably through the production of catechol metabolites via hydroxylation at the 2 or 4 position (Cavalieri et al. 2002; Wellejus et al. 2004) as cited in Rempel-Hester et al. (2009)...
    • ...It has been reported that E2 can enhance DNA damage in fish (Maria et al. 2008; Rempel-Hester et al. 2009)...

    Harikrishnan Thilagamet al. 17β estradiol induced ROS generation, DNA damage and enzymatic respons...

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