Academic
Publications
High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena

High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena,10.1007/s10535-010-0041-z,Biologia Plantarum,Y

High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena   (Citations: 2)
BibTex | RIS | RefWorks Download
A novel protocol for plant regeneration from cotyledon explants of eggplant (Solanum melongena) reducing concentration of sucrose was established. The most efficient bud induction medium consisted of Murashige and Skoog (MS) medium supplemented with 2.0 mg dm−3 zeatin, 0.1 mg dm−3 indoleacetic acid and 10 g dm−3 sucrose. After 15 d, the shoot buds were fragmented and transferred to the shoot elongation MS supplemented with 1.0–2.0 mg dm−3 gibberellic acid and 4.0–8.0 mg dm−3 AgNO3, which promoted shoots elongation. The genetic stability of the regenerated plants was analyzed by flow cytometry, RAPD and SSR molecular markers. The results indicated that almost no somaclonal variation was detected among the regenerants.
Journal: Biologia Plantarum - BIOL PLANT , vol. 54, no. 2, pp. 231-236, 2010
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
    • ... plant regeneration from root explants was reported for Populus tremula (Vinocur et al. 2000), Lycopersicon sp. (Peres et al. 2001), Diospyros kaki (Carvalho and Biasi 2004), Helianthus annuus 9 H. tuberosus (Fambrini et al. 2003), Melia azedarach (Vila et al. 2005), Elentherococcus koreanum (Park et al. 2005), Arabidopsis (Atta et al. 2009), Azadirachta indica (Arora et al. 2010), Cleome rosea (Simo ˜es et al. 2009), Solanum melongena (Xing et ...
    • ...Flow cytometric analysis has been very useful to detect changes in ploidy levels in tissue, cell and organ culture of plants (Loureiro et al. 2005; Dolez ÿel et al. 2007a, b; Clarindo et al. 2008; Brito et al. 2010; Xing et al. 2010, Yang et al. 2010)...

    Crislene Viana da Silvaet al. Organogenesis from root explants of commercial populations of Passiflo...

Sort by: