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Fast Isolation of Highly Active Photosystem II Core Complexes from Spinach

Fast Isolation of Highly Active Photosystem II Core Complexes from Spinach,10.1111/j.1744-7909.2010.00971.x,Journal of Integrative Plant Biology,Zhao-

Fast Isolation of Highly Active Photosystem II Core Complexes from Spinach   (Citations: 1)
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    • ... have been used to prepare PSII reaction centre cores from higher plants which include multiple steps membrane solubilization with rather long exposure to different non-ionic detergents (i.e., Triton X-100, n-octyl beta-D-glucopyranoside, beta-dodecylmaltoside (b-DM), digitonin) followed by a final isolation step through sucrose density centrifugation (Ikeuchi et al. 1985; Bricker et al. 1985; Haag et al. 1990; Hankamer et al. 1997b; Wang et ...
    • ...Compared to other PSII cores depleted of LHCII and isolated by sucrose gradient (Ikeuchi et al. 1985; Haag et al. 1990; Hankamer et al. 1997b; Wang et al. 2010), the pea PSII core complex reported here showed a higher integrity in the polypeptide composition of the OEC: an almost stoichiometric presence of PsbO and PsbR was confirmed, as well as a detectable amount of PsbP (Fig. 3b)...
    • ...We included glycine betaine in our buffers during the isolation of thylakoids and PSII for the purpose of stabilizing the OEC extrinsic proteins (Murata et al. 1992; Papageorgiou and Murata 1995; Catucci et al. 1998; Wang et al. 2010)...

    Cristina Paglianoet al. One-step isolation and biochemical characterization of a highly active...

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