Monoclonal Antibodies against Pig Ovarian Follicular Granulosa Cells Induce Apoptotic Cell Death in Cultured Granulosa Cells

...Previously, we prepared an IgM monoclonal antibody (named PFG-1) capable of inducing granulosa cell apoptosis (Myoumoto et al., 1997; Manabe et al., 1997c, 1998a, b). PFG-1 was produced against granulosa cells prepared from healthy antral follicles of porcine ovaries, and specifically recognized a cellmembrane glycoprotein (PFG-1 antigen) with a molecular weight of 55 kD and isoelectric point of 5.9...
...Granulosa cells from healthy antral follicles of porcine ovaries were prepared as described previously (Myoumoto et al., 1997)...
...Then, antibody class was determined by ELISA as described previously (Myoumoto et al., 1997)...
...For conventional Western blotting analysis, homogenized samples of ovarian tissues (granulosa cells and luteal bodies), oviduct, uterus, testis, liver, kidney, adrenal gland, pancreas, stomach, small intestine, large intestine, spleen, thymus, brain, heart, lung or skeletal muscle were electrophoresed through 4% polyacrylamide slab gels as described previously (Myoumoto et al., 1997)...
...As previously reported (Myoumoto et al., 1997), cell membrane samples of the isolated granulosa cells were separated by two-dimensional PAGE (2D-PAGE) which was performed according to the method of O’Farrell (1975)...
...The trypan blue exclusion method was used to determine cell-killing activity (Myoumoto et al., 1997)...
... follicles (lanes 3 and 5). In the granulosa cell samples prepared from atretic follicles, a specific band with a molecular weight of 42 kD was observed on the nitrocellulose filters treated with PFG-3 and PFG-4, but the band of 55 kD disappeared (lanes 4 and 6). In addition, when the nitrocellulose filters were treated with PFG-1, which is an IgM monoclonal antibody capable of inducing granulosa cell apoptosis (Manabe et al., 1997b; Myoumoto ...
...Previously, we generated a unique monoclonal IgM antibody, named PFG-1, and 2D-Western blotting analysis showed that this antibody recognized a cell membrane protein (55 kD, pI 5.9, named PFG-1 antigen) of porcine granulosa cells (Manabe et al., 1997c; Myoumoto et al., 1997)...
...Similarly to one of PFG- 3-recognized antigen (55 kD, pI 5.9), PFG-1 antigen was immunochemically and immunohistochemically detected only in the granulosa cells of healthy but not atretic follicles (Myoumoto et al., 1997)...

Noboru Manabe, et al. Immunochemical characteristics of a novel cell death receptor and a de...

Keywords (3)

Monoclonal Antibodies against Pig Ovarian Follicular Granulosa Cells Induce Apoptotic Cell Death in Cultured Granulosa Cells,10.1292/jvms.59.641,Journ

Citation Context (1)

Noboru Manabe, et al. Immunochemical characteristics of a novel cell death receptor and a de...

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Involvement of fas antigen in ovarian follicular atresia and luteolysis (Citations: 45)

Multiple Pathways for Apoptotic Nuclear Fragmentation (Citations: 78)

Identification of Deoxyribonuclease II as an Endonuclease Involved in Apoptosis (Citations: 158)

Detection of Mg2+Dependent Endonuclease Activity in Myeloid Leukemia Cell Nuclei Capable of Producing Internucleosomal DNA Cleavage (Citations: 15)

Ca2+/Mg2+Dependent Endonuclease but not Ca22+Dependent, Mg2+Dependent or Cation-lndependent Endonuclease is Involved in Granulosa Cell Apoptosis of Pig Atretic Follicles (Citations: 12)

Citations (9)

Regulation Mechanism of Selective Atresia in Porcine Follicles: Regulation of Granulosa Cell Apoptosis during Atresia (Citations: 26)

TRAIL-Decoy Receptor1 Disappears in Granulosa Cells of Atretic Follicles in Porcine Ovaries (Citations: 10)

TRADD is Involved in Apoptosis Induction in Granulosa Cells during Atresia in Pig Ovaries (Citations: 6)

TRAIL-Decoy Receptor 1 Plays Inhibitory Role in Apoptosis of Granulosa Cells from Pig Ovarian Follicles (Citations: 8)

Changes in Localization of Type I, III and IV Collagens and Their mRNA Expression During Follicular Atresia in Bovine and Porcine Ovaries (Citations: 2)


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