Academic
Publications
An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation

An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation,10.1038/nature09025,Nature,Zhihuan Gao,Hai-Liang Liu,Lucia Daxi

An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation   (Citations: 21)
BibTex | RIS | RefWorks Download
Zhihuan Gao, Hai-Liang Liu, Lucia Daxinger, Olga Pontes, Xinjian He, Weiqiang Qian, Huixin Lin, Mingtang Xie, Zdravko J. Lorkovic, Shoudong Zhang, Daisuke Miki, Xiangqiang Zhanhttp://academic.research.microsoft.com/io.ashx?type=5&id=45914252&selfId1=0&selfId2=0&maxNumber=12&query=
DNA methylation is an important epigenetic mark in many eukaryotes. In plants, 24-nucleotide small interfering RNAs (siRNAs) bound to the effector protein, Argonaute 4 (AGO4), can direct de novo DNA methylation by the methyltransferase DRM2 (refs 2, 4-6). Here we report a new regulator of RNA-directed DNA methylation (RdDM) in Arabidopsis: RDM1. Loss-of-function mutations in the RDM1 gene impair the accumulation of 24-nucleotide siRNAs, reduce DNA methylation, and release transcriptional gene silencing at RdDM target loci. RDM1 encodes a small protein that seems to bind single-stranded methyl DNA, and associates and co-localizes with RNA polymerase II (Pol II, also known as NRPB), AGO4 and DRM2 in the nucleus. Our results indicate that RDM1 is a component of the RdDM effector complex and may have a role in linking siRNA production with pre-existing or de novo cytosine methylation. Our results also indicate that, although RDM1 and Pol V (also known as NRPE) may function together at some RdDM target sites in the peri-nucleolar siRNA processing centre, Pol II rather than Pol V is associated with the RdDM effector complex at target sites in the nucleoplasm.
Journal: Nature , vol. 465, no. 7294, pp. 106-109, 2010
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
Sort by: