Strain improvement in actinomycetes in the postgenomic era

Strain improvement in actinomycetes in the postgenomic era,10.1007/s10295-010-0934-z,Journal of Industrial Microbiology & Biotechnology,Richard H. Bal

Strain improvement in actinomycetes in the postgenomic era   (Citations: 2)
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With the recent advances in DNA sequencing technologies, it is now feasible to sequence multiple actinomycete genomes rapidly and inexpensively. An important observation that emerged from early Streptomyces genome sequencing projects was that each strain contains genes that encode 20 or more potential secondary metabolites, only a fraction of which are expressed during fermentation. More recently, this observation has been extended to many other actinomycetes with large genomes. The discovery of a wealth of orphan or cryptic secondary metabolite biosynthetic gene clusters has suggested that sequencing large numbers of actinomycete genomes may provide the starting materials for a productive new approach to discover novel secondary metabolites. The key issue for this approach to be successful is to find ways to turn on or turn up the expression of cryptic or poorly expressed pathways to provide material for structure elucidation and biological testing. In this review, I discuss several genetic approaches that are potentially applicable to many actinomycetes for this application.
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    • ...With the advent of rapid and inexpensive DNA sequencing, many actinomycete genomes can now be surveyed for known and cryptic secondary metabolite biosynthetic gene clusters, and novel pathways can be expressed using a variety of approaches [4, 5, 7, 10, 14, 60]...
    • ...These in turn may be useful in targeting potentially functional, but otherwise cryptic NRPS and other NRPS-like pathways for expression studies [5, 7, 60]...
    • ...Heterologous expression. There are a number of strains that may be useful for the heterologous expression of cryptic gene clusters identified in genome sequencing projects [5, 7]. In some of these strains, certain secondary metabolite biosynthetic gene clusters have been deleted to facilitate the channeling of precursors into the heterologous pathway and to simplify the identification of novel metabolites...

    Richard H. Baltz. Function of MbtH homologs in nonribosomal peptide biosynthesis and app...

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