Academic
Publications
Biocalorimetry as a process analytical technology process analyser; robust in-line monitoring and control of aerobic fed-batch cultures of crabtree-negative yeast cells

Biocalorimetry as a process analytical technology process analyser; robust in-line monitoring and control of aerobic fed-batch cultures of crabtree-ne

Biocalorimetry as a process analytical technology process analyser; robust in-line monitoring and control of aerobic fed-batch cultures of crabtree-negative yeast cells   (Citations: 2)
BibTex | RIS | RefWorks Download
Control of bioprocesses requires reliable and robust on- or in-line monitoring tools providing real-time information on process dynamics. Heat generation related to metabolic activity of living systems is currently gaining importance in bioprocess industry due to its non-invasive and essentially instantaneous characteristics. This study deals with monitoring and control of pure aerobic fed-batch cultures of three Crabtree-negative yeast strains, Kluyveromyces marxianus, Candida utilis and Pichia pastoris, based on in-line measured, metabolic heat flow signals. A high resolution biocalorimeter (BioRC1) was developed from a standard bench-scale heat flow calorimeter (RC1). The BioRC1 was equipped with in-line (dielectric spectroscopy, pH probe and dissolved oxygen probe) and at-line (exit gas analyser) sensors to characterise the growth behaviour of the yeast cells. Both metabolic heat flow and biomass profiles exhibited similar behaviour proving the significance of employing heat flow signal as a key-parameter for the system under investigation. A simple estimator for biomass concentration and specific growth rate was formulated based on heat flow values. In order to evaluate the potential of calorimetry as a reliable and powerful process monitoring tool, the robustness, reliability as well as the broad applicability of the developed estimators was assessed through comparison with off-line measurement techniques and showed promising results for general applicability with a wide range of bioprocesses.
Journal: Journal of Thermal Analysis and Calorimetry - J THERM ANAL CALORIM , vol. 104, no. 1, pp. 75-85, 2011
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
    • ...1 and 2 ,h as been described in previous work (Sivaprakasam et al. 2011)...
    • ...The applicability of biocalorimetry as a monitoring tool for biomass in microbial cultures has been assessed in previous work (Sivaprakasam et al. 2011) for the three yeast strains under study and is illustrated in Fig. 2 for a batch culture of C. utilis...
    • ...The present work confirmed the reliability of biocalorimetry as a biomass monitoring device (Sivaprakasam et al. 2011) and established the potential of the Appl Microbiol Biotechnol...

    Moira Monika Schuleret al. Investigation of the potential of biocalorimetry as a process analytic...

Sort by: