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Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat

Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat,10.1007/s11105-010-0249-2,Plant Molecular

Suppression Subtractive Hybridization Identified Differentially Expressed Genes in Pistil Mutations in Wheat   (Citations: 2)
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To understand the molecular mechanism of the three pistils mutation in wheat, two forward subtractive cDNA libraries from two pairs of near-isogenic wheat lines, Chuanmai 28 three pistils (CMTP) and Chinese Spring three pistils (CSTP), were constructed using SSH. A total of 68 clones in CMTP lines and 197 clones in CSTP lines were identified as potentially over-expressed clones. Thirty-two out of 68 clones in CMTP lines belonged to unknown proteins; while, the remaining 30 clones shared homology to diverse classes of genes involved in protein modulation and protein synthesis, signal transduction, and ion transporters. Approximately 67% of genes in CSTP lines were either unclassified or had no matches (“no hits”) in the database and about 33% of identified genes encoded polypeptides with known functions. Sequence comparisons of cDNA clones between the two forward cDNA libraries revealed that four genes, encoding thioredoxin H, ubiquitin protein ligases, MCM2, and ubiquinol-cytochrome C reductase complex 14 kDa proteins, were over-expressed in both libraries. These genes would likely play an important role in determining the three pistils trait in the mutant wheat line.
Journal: Plant Molecular Biology Reporter - PL MOL BIOL REP , vol. 29, no. 2, pp. 431-439, 2011
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