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Mechanisms of Bacillus cereus biofilm formation: an investigation of the physicochemical characteristics of cell surfaces and extracellular proteins

Mechanisms of Bacillus cereus biofilm formation: an investigation of the physicochemical characteristics of cell surfaces and extracellular proteins,1

Mechanisms of Bacillus cereus biofilm formation: an investigation of the physicochemical characteristics of cell surfaces and extracellular proteins   (Citations: 2)
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Microbial biofilms contribute to biofouling in a wide range of processes from medical implants to processed food. The extracellular polymeric substances (EPS) are implicated in imparting biofilms with structural stability and resistance to cleaning products. Still, very little is known about the structural role of the EPS in Gram-positive systems. Here, we have compared the cell surface and EPS of surface-attached (biofilm) and free-floating (planktonic) cells of Bacillus cereus, an organism routinely isolated from within biofilms on different surfaces. Our results indicate that the surface properties of cells change during biofilm formation and that the EPS proteins function as non-specific adhesions during biofilm formation. The physicochemical traits of the cell surface and the EPS proteins give us an insight into the forces that drive biofilm formation and maintenance in B. cereus.
Journal: Applied Microbiology and Biotechnology - APPL MICROBIOL BIOTECHNOL , vol. 89, no. 4, pp. 1161-1175, 2011
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    • ...2005; Eboigbodin and Biggs 2008; Flemming and Wingender 2010; Karunakaran and Biggs 2011)...

    Joy Mukherjeeet al. Using a multi-faceted approach to determine the changes in bacterial c...

    • ...FTIR spectroscopy of EPS extracted from disrupted biofilms has also been carried out (Eboigbodin and Biggs 2008; Tapia et al. 2009; Liang et al. 2010; Karunakaran and Biggs 2011)...
    • ...A recent work on the detailed biochemical analysis of the protein components of EPS attributes a small yet significant contribution of polycationic peptides in maintaining the structural integrity of Bacillus cereus biofilms (Karunakaran and Biggs 2011)...
    • ...The EPS molecules, by a process called polymer bridging, have also been found to play an important role in overcoming the electrostatic repulsion between the bacterium and the surface, thus ensuring firm, irreversible attachment of the bacteria to the surface (Neu and Marshall1990; Karunakaran and Biggs 2011)...
    • ...Karunakaran and Biggs (2011) have shown that there are distinct differences in the cell surface characteristics of the adhered versus the free-floating cells...

    Esther Karunakaranet al. “Biofilmology”: a multidisciplinary review of the study of microbial b...

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