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Patterns of Gram-Stained Fecal Flora as a Quick Diagnostic Marker in Patients with Severe SIRS

Patterns of Gram-Stained Fecal Flora as a Quick Diagnostic Marker in Patients with Severe SIRS,10.1007/s10620-010-1486-9,Digestive Diseases and Scienc

Patterns of Gram-Stained Fecal Flora as a Quick Diagnostic Marker in Patients with Severe SIRS   (Citations: 2)
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Kentaro Shimizu, Hiroshi Ogura, Kazunori Tomono, Osamu Tasaki, Takashi Asahara, Koji Nomoto, Masami Morotomi, Asako Matsushima, Yasutaka Nakahori, Shuhei Yamano, Akinori Osuka, Yasuyuki Kuwagatahttp://academic.research.microsoft.com/io.ashx?type=5&id=48488468&selfId1=0&selfId2=0&maxNumber=12&query=
Background  The gut is an important target organ of injury during critically ill conditions. Although Gram staining is a common and quick method for identifying bacteria, its clinical application has not been fully evaluated in critically ill conditions. Aims  This study’s aims were to identify patterns of Gram-stained fecal flora and compare them to cultured bacterial counts and to investigate the association between the patterns and septic complications in patients with severe systemic inflammatory response syndrome (SIRS). Methods  Fifty-two patients with SIRS were included whose Gram-stained fecal flora was classified into three patterns. In a diverse pattern, large numbers of multiple kinds of bacteria completely covered the field. In a single pattern, one specific kind of bacteria or fungi predominantly covered the field. In a depleted pattern, most bacteria were diminished in the field. Results  In the analysis of fecal flora, the numbers of total obligate anaerobes in the depleted pattern was significantly lower than those in the diverse pattern and single pattern (p < 0.05). The concentrations of total organic acids, acetic acid, and propionic acid in the depleted pattern were significantly lower than those in diverse pattern and single pattern (p < 0.05). Mortality due to multiple organ dysfunction syndrome for the single pattern (52%) and the depleted pattern (64%) was significantly higher than that for the diverse pattern (6%) (p < 0.05). Conclusions  Gram-stained fecal flora can be classified into three patterns and are associated with both cultured bacterial counts and clinical information. Gram-stained fecal bacteria can be used as a quick bedside diagnostic marker for severe SIRS patients.
Journal: Digestive Diseases and Sciences - DIGEST DIS SCI , vol. 56, no. 6, pp. 1782-1788, 2011
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    • ...Shimizu et al. reported that gut flora depletion, and loss of obligate anaerobes in particular, increases the risk of gut-derived sepsis, enteritis, and mortality [4, 9]. Changed gut flora and impaired intestinal barrier function are thought to promote the movement of endotoxin and gut bacteria into the portal circulation and gut lymphatics [7]...
    • ...Simple methods such as fecal gram staining [9] have the ability to identify microbiota depletion and can be used in the emergency department...

    Joe Alcock. Dangerous Disappearing Act: Commensal Gut Microbiota After Acute Sever...

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