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A Simple and Sensitive Resonance Scattering Spectral Assay for Detection of Melamine Using Aptamer-Modified Nanosilver Probe

# A Simple and Sensitive Resonance Scattering Spectral Assay for Detection of Melamine Using Aptamer-Modified Nanosilver Probe,10.1007/s11468-011-9215-6

A Simple and Sensitive Resonance Scattering Spectral Assay for Detection of Melamine Using Aptamer-Modified Nanosilver Probe
Nanosilver of 10-nm size was prepared by the NaBH4–sodium citrate procedure, and it was modified by a single-strand DNA (ssDNA) aptamer to fabricate an AgssDNA probe for melamine. The probe was stabile at pH 7.0 Na2HPO4–NaH2PO4 buffer solutions and in the presence of 25.0 mmol/L NaCl. Upon the addition of melamine, it interacted with the probe to aggregate big clusters, which led to the resonance scattering (RS) intensity at 470 nm increasing greatly. Under the selected conditions, the increased RS intensity (ΔI 470 nm) is linear to melamine concentration in the range of 6.31–378.4 μg/L, with a regression equation of $$\Delta {I_{{47}0{\rm{ nm}}}} = {1}.{124}c + { 10}.{8}$$ and a detection limit of 3.1 μg/L. The aptamer-modified nanosilver RS assay has been applied for the determination of melamine in milk, with satisfactory results.