Development of EST-based new SSR markers in seabuckthorn

Development of EST-based new SSR markers in seabuckthorn,10.1007/s12298-010-0037-3,Physiology and Molecular Biology of Plants,Ankit Jain,Rajesh Ghanga

Development of EST-based new SSR markers in seabuckthorn   (Citations: 2)
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EST-based SSR markers were developed by screening a collection of 1584 clustered ESTs of seabuckthorn (Hippophae rhamnoides). PCR primers were designed for the amplification of 30 microsatellite loci. Two to five allelic bands were displayed by nine primer pairs in H. rhamnoides genotypes and by eleven primer pairs in H. salicifolia genotypes. None of the thirty primer pairs detected polymorphism in H. tibetana genotypes. Considering the high polymorphism detected in the tested genotypes and their direct origin from the genic regions, these EST-SSR markers hold immense promise in seabuckthorn genome analysis, molecular breeding and population genetics.
Journal: Physiology and Molecular Biology of Plants , vol. 16, no. 4, pp. 375-378, 2010
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    • ...In the last decade, interest in sea buckthorn’s health benefits has increased considerably in many countries. Although several plantations have come up in Canada, there is limited information on the metabolite composition of the currently grown cultivars. Furthermore, this plant is currently an unexplored source of novel traits and genes. So far, only about 2787 high quality sequences (>100 bp in length) derived from a cDNA library prepared from leaf tissue have been submitted to the EST database (dbEST) of NCBI ...

    Tahira Fatimaet al. Fatty Acid Composition of Developing Sea Buckthorn (Hippophae rhamnoid...

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