Pressure induced nucleus DNA fragmentation

Pressure induced nucleus DNA fragmentation,10.1007/s10815-010-9525-1,Journal of Assisted Reproduction and Genetics,Cezary Grygoruk,Piotr Sieczynski,Ja

Pressure induced nucleus DNA fragmentation   (Citations: 1)
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Purpose  The present study was designed to investigate the impact of pressure on nuclear DNA integrity in viable cells of mouse blastocysts. Methods  The blastocysts of hybrid F1 females [(C57Bl/10 J × CBA-H);N = 15] aged 2–3 months were exposed into the pressure impulse lasting ~0.021 s and characterized by a positive pressure peak of ~76 mmHg. The nuclear DNA fragmentation index of mouse blastocysts was assessed by TUNEL assay within 60 s after exposure to pressure impulse. Results  The mean nuclear DNA fragmentation index was significantly higher in the experimental group (83%) than in the control group (19.7%); p < 0.001. Conclusion(s)  A low magnitude pressure impulse can induce nuclear DNA fragmentation in mouse blastocysts. The compression and decompression forces appearing during pressure fluctuations are responsible for the observed DNA shearing.
Journal: Journal of Assisted Reproduction and Genetics - J ASSIST REPROD GENET , vol. 28, no. 4, pp. 363-368, 2011
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