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Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV

Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV,10.1016/j.cimid.2010.11

Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV   (Citations: 1)
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In the present study, we describe the development of a DNA vaccine against chicken anemia virus. The VP1 and VP2 genes of CAV were amplified and cloned into pBudCE4.1 to construct two DNA vaccines, namely, pBudVP1 and pBudVP2-VP1. In vitro and in vivo studies showed that co-expression of VP1 with VP2 are required to induce significant levels of antibody against CAV. Subsequently, the vaccines were tested in 2-week-old SPF chickens. Chickens immunized with the DNA-plasmid pBudVP2-VP1 showed positive neutralizing antibody titer against CAV. Furthermore, VP1-specific proliferation induction of splenocytes and also high serum levels of Th1 cytokines, IL-2 and IFN-γ were detected in the pBudVP2-VP1-vaccinated chickens. These results suggest that the recombinant DNA plasmid co-expressing VP1 and VP2 can be used as a potential DNA vaccine against CAV.
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    • ...The assay was carried out by the measurement of cell proliferation and viability using WST-1 reagent (Roche, Germany) as described by Moeini et al. [12] and Lehtoranta et al. [13]...
    • ...According to the previous studies [6,14,15] and the finding of our group [12], co-synthesis of the VP1 and VP2 proteins is required to produce the essential neutralizing form of VP1 resulting in the efficient induction of antibody response to CAV, whereas separate expression of VP1 does not...

    Hassan Moeiniet al. Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV...

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