Academic
Publications
Effect of reactive oxygen and carbonyl species on crucial cellular antioxidant enzymes

Effect of reactive oxygen and carbonyl species on crucial cellular antioxidant enzymes,10.1016/j.cbi.2010.12.028,Chemico-biological Interactions,Jean-

Effect of reactive oxygen and carbonyl species on crucial cellular antioxidant enzymes   (Citations: 1)
BibTex | RIS | RefWorks Download
Numerous reactive oxygen species (ROS) and reactive carbonyl species (RCS) issuing from lipid and sugar oxidation are known to damage a large number of proteins leading to enzyme inhibition and alteration of cellular functions. Whereas studies in literature only focus on the reactivity of one or two of these compounds, we aimed at comparing in the same conditions of incubations (4 and 24h at 37°C) the effects of both various RCS (4-hydroxynonenal, 4-hydroxyhexenal, acrolein, methylglyoxal, glyoxal, malondialdehyde) and ROS (H2O2, AAPH) on the activity of key enzymes involved in cellular oxidative stress: superoxide dismutase (Cu,Zn-SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST) and glucose-6-phosphate dehydrogenase (G6PDH). This was realized both in vitro on purified proteins and MIAPaCa-2 cells. Incubation of these enzymes with RCS resulted in a significant time- and concentration-dependent inhibition for both pure enzymes and in cell lysates. Among all RCS and ROS, hydroxynonenal (HNE) was observed as the most toxic for all studied enzymes except for SOD and is followed by hydrogen peroxide. At 100μM, HNE resulted in a 50% reduction of GPx, 56% of GST, 65% of G6PDH, and only 10% of Cu,Zn-SOD. Meanwhile it seems that concentrations used in our study are closer to biological conditions for ROS than for RCS. H2O2 and AAPH-induced peroxyl radicals may be probably more toxic towards the studied enzymes in vivo.
Journal: Chemico-biological Interactions - CHEM-BIOL INTER , vol. 190, no. 1, pp. 28-34, 2011
Cumulative Annual
View Publication
The following links allow you to view full publications. These links are maintained by other sources not affiliated with Microsoft Academic Search.
Sort by: