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New genetic polymorphisms of KiSS1 gene and their association with litter size in goats

New genetic polymorphisms of KiSS1 gene and their association with litter size in goats,10.1016/j.smallrumres.2010.11.013,Small Ruminant Research,J. X

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New genetic polymorphisms of KiSS1 gene and their association with litter size in goats   (Citations: 1)
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In the present study, the polymorphisms of kisspeptin (KiSS-1) gene were analyzed in 652 individuals from three goat breeds: Xinong Saanen goats (SG), Guanzhong goats (GZ) and Boer goats (BG). Three alleles (T, C and G) and four genotypes (TT, TC, CC and TG) were detected in the intron 2 of KiSS-1 gene by PCR-SSCP analysis. In addition, two single nucleotide polymorphisms (SNPs) – T2643C and 8bp base deletions (2677AGTTCCCC) were identified by DNA sequencing. The SNPs loci were in Hardy–Weinberg disequilibrium in three goat breeds (P<0.05). After comparing genotypic distribution within three goat breeds, BG had conspicuous differences from SG and GZ (P<0.001). Association of polymorphism with litter size was done in SG and GZ breeds, which showed to be associated with litter size in both goat breeds. The SNP in goat KiSS-1 gene had significant effects on litter size (P<0.05). Therefore, these results suggest that KiSS-1 gene is a strong candidate gene that affects litter size in goats.
Journal: Small Ruminant Research - SMALL RUMINANT RES , vol. 96, no. 2, pp. 106-110, 2011
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