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Keywords
(8)
Bacillus Subtilis
Farmyard Manure
Field Experiment
Organic Amendment
Organic Manure
pseudomonas fluorescens
Root Rot
Zinc
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Combining Pseudomonas, Bacillus and Trichoderma strains with organic amendments and micronutrient to enhance suppression of collar and root rot disease in physic nut
Combining Pseudomonas, Bacillus and Trichoderma strains with organic amendments and micronutrient to enhance suppression of collar and root rot diseas
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Combining Pseudomonas, Bacillus and Trichoderma strains with organic amendments and micronutrient to enhance suppression of collar and root rot disease in physic nut
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P. Latha
,
T. Anand
,
V. Prakasam
,
E. I. Jonathan
,
M. Paramathma
,
R. Samiyappan
The fungal and bacterial biocontrol agents were tested individually and in combinations with oil cakes, organic manures and micronutrients for their efficacy against collar and
root rot
pathogen, Lasiodiplodia theobromae under in vitro, glasshouse and field conditions. Among the fungal (Trichoderma) and bacterial (Pseudomonas and Bacillus) antagonists screened against L. theobromae under in vitro conditions, Trichoderma viride (Tv1),
Pseudomonas fluorescens
(Pf1) and
Bacillus subtilis
(Bs16) isolates exhibited maximum inhibition compared to other isolates. Among the oil cakes, organic manures and micronutrients tested in vitro against the pathogen, neem cake,
farmyard manure
(FYM) and
zinc
sulphate were most effective in reducing the growth of the pathogen. The compatibility studies revealed the isolate of T. viride (Tv1), P. fluorescens (Pf1) and B. subtilis (Bs16) were compatible with other and also with neem cake and
zinc
sulphate. Of the biocontrol agents tested individually as well as in mixtures with neem cake, FYM and
zinc
sulphate against L. theobromae, combination of Pf1+Tv1+Bs16+neem cake+FYM+zinc sulphate showed maximum inhibition in mycelial growth of the pathogen. In glasshouse and field experiments also, the mixture of Pf1+Tv1+Bs16+neem cake+FYM+zinc sulphate was found to be superior in reducing the collar and
root rot
disease incidence compared to other treatments.
Journal:
Applied Soil Ecology - APPL SOIL ECOL
, vol. 49, pp. 215-223, 2011
DOI:
10.1016/j.apsoil.2011.05.003
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