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Keywords
(8)
Detection Limit
Dopamine
Electrochemical Impedance Spectroscopy
Environmental Pollution
Quartz Crystal Microbalance
Sulfate Reducing Bacteria
Electron Transfer
High Density
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Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform
Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform,10.1016/j.bios.2010.11.013,Biosensors & Bioelectronics,Yi Wan,
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Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform
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Yi Wan
,
Dun Zhang
,
Yi Wang
,
Peng Qi
,
Baorong Hou
A sensitive and selective immunosensor for the nonlabeled detection of sulfate-reducing bacteria (SRB) is constructed using a self-polymerised polydopamine film as the immobilisation platform. Self-polymerisation of
dopamine
is used as a powerful approach for applying multifunctional coatings onto the surface of a gold electrode. The polydopamine film is used not only as the immobilisation platform, but also as a cross-linker reagent for the immobilisation of the anti-SRB antibody. The polydopamine film is loaded with a
high density
of anti-SRB antibodies linked to the substrate to obtain high response signals. The formation and fabrication of the biosensor and the quantification of antibody anchoring are monitored, and SRB detection is performed by either
quartz crystal microbalance
(QCM) or
electrochemical impedance spectroscopy
(EIS). After modeling the impedance Nyquist plots of the SRB/anti-SRB/polydopamine/gold electrode for increasing concentrations of SRB, the
electron transfer
resistance (Rct) is used as a measure of immunocomplex binding. The Rct is correlated with the concentration of bacterial cells in the range of 1.8×102 to 1.8×106CFUmL−1; the
detection limit
is 50CFUmL−1. This work demonstrates a new immobilisation platform for the development of a sensitive and label-less impedimetric and piezoelectric immunosensor. This immunosensor may be broadly applied in clinical diagnoses and the monitoring of water environmental pollution. The method proposed is distinct in its ease of application, use of a simple protocol, and mild reaction conditions. These allow it to be applied to a wide variety of materials.
Journal:
Biosensors & Bioelectronics - BIOSENS BIOELECTRON
, vol. 26, no. 5, pp. 2595-2600, 2011
DOI:
10.1016/j.bios.2010.11.013
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