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Systematic evaluation of pre-HPLC sample processing methods on total and individual isoflavones in soybeans and soy products

Systematic evaluation of pre-HPLC sample processing methods on total and individual isoflavones in soybeans and soy products,10.1016/j.foodres.2010.12

Systematic evaluation of pre-HPLC sample processing methods on total and individual isoflavones in soybeans and soy products   (Citations: 1)
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There are mainly two protocols in isoflavone analysis, one that involves hydrolysis prior to HPLC analysis and the other direct HPLC analysis. In this study, three different hydrolysis methods were systematically re-evaluated, and compared with direct HPLC analysis. Acidic hydrolysis (1.2–3M HCl in ethanol at 80°C) showed a maximum conversion of ca. 92% from glucosides to aglycones in 2h; however, longer reaction caused degradation of genistein. Alkaline hydrolysis using 2M NaOH converted acetylglucosides and malonylglucosides to their respective glucosides within 10min. Glucuronidase from H. pomatia effectively converted isoflavone glucosides and acetylglucosides to aglycones. Quantification of the total isoflavones in various soy food products showed no significant difference among direct injection and the three hydrolysis methods (P<0.05). We conclude that direct analysis of isoflavones in crude extracts is a rapid and accurate method to obtain isoflavone profiles and compositions in soybean, soy foods and beverages.
Journal: Food Research International - FOOD RES INT , vol. 44, no. 8, pp. 2425-2434, 2011
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